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Oligo Synthesis

Oligo Synthesis : Supports,CPGs

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3'-dA-CPG

3'-dA-CPG

Glen Research

Catalogue No.DescriptionPack SizePriceQty
20-2004-013'-dA-CPG 0.1g £320.00£294.40Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2104-413'-dA-CPG 4x1.0µm £540.00£496.80Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2104-423'-dA-CPG 4x0.2µm £180.00£165.60Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

3'-dA-CPG

3'-dA-CPG

Glen Research

3'-dA-CPG

Structure

Catalog Number: 20-2004-xx

(20-2104-xx)

Description: 3'-dA-CPG

5'-Dimethoxytrityl-N-benzoyl-3'-deoxyAdenosine,
2'-succinoyl-long chain alkylamino-CPG
    F.W.: 313.21

Diluent: Not Applicable
Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
Deprotection: Deprotect using the protocol required by the nucleobases.
Storage: Controlled room temperature or lower, dry
Stability in Solution: Not Applicable

chain terminators

In situations where ligation must be blocked at the 5’ terminus, 5’-OMe-dT may be used. 5’-OMe modification of a strand of siRNA using 5’-OMe-T can control guide strand selection and targeting specificity.1 5’-Amino-dT terminates an oligonucleotide with a 5’-amino group which may be used for attaching a peptide or a PNA sequence. To avoid polymerase extension at the 3’ terminus, 2’,3’-dideoxynucleoside and 3’-deoxynucleoside CPGs have proved to be effective. 2’,3’- Phosphoramidites are designed to be used with the 5’-phosphoramidites and supports. Since these phosphoramidites have no DMT group, they are not compatible with purification by the DMT-on technique. Ion exchange HPLC or PAGE should be used to purify these dideoxy terminated oligos to ensure that shorter sequences (containing 3’-OH) groups are removed. (3’-Termination can also be effected using a 3’-3’ linkage formed using 5’-supports, or 3’-spacer C3 CPG.)

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

3'-dA-CPG

3'-dA-CPG

Glen Research

MSDS

Glen Report 8.1

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

3'-dA-CPG

3'-dA-CPG

Glen Research

To avoid polymerase extension at the 3’ terminus, 2’,3’-dideoxynucleoside and 3’-deoxynucleoside CPGs have proved to be effective.  2’,3’-phosphoramidites are designed to be used with the 5’-phosphoramidites and supports.  Since these phosphoramidites have no DMT group, they are not compatible with purification by the DMT-on technique.  Ion exchange HPLC or PAGE should be used to purify these dideoxy terminated oligos to ensure that shorter sequences (containing 3’-OH) groups are removed.  (3’-termination can also be effected using a 3’-3’ linkage formed using 5’-supports, or 3’-spacer C3 CPG.)

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200