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Oligo Synthesis

Oligo Synthesis : Supports,CPGs

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3'-Phosphate CPG

3'-Phosphate CPG

Glen Research

Catalogue No.DescriptionPack SizePriceQty
20-2900-013'-Phosphate CPG 0.1g £56.00£51.52Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2900-103'-Phosphate CPG 1.0g £384.00£353.28Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2900-133'-Phosphate CPG 1x10µm £144.00£132.48Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2900-143'-Phosphate CPG 1x15µm £224.00£206.08Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2900-413'-Phosphate CPG 4x1.0µm £80.00£73.60Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2900-423'-Phosphate CPG 4x0.2µm £48.00£44.16Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

3'-Phosphate CPG

3'-Phosphate CPG

Glen Research

3'-Phosphate CPG

 

Structure

 

Catalog Number: 20-2900-xx

Description: 3'-Phosphate CPG

2-[2-(4,4'-Dimethoxytrityloxy)ethylsulfonyl]ethyl-2-succinoyl)-
long chain alkylamino-CPG


F.W.: 79.98

Diluent: Not Applicable
The 3'-Phosphate supports are not compatible with Β-elimination techniques such as 10% diethylamine in acetonitrile to remove cyanoethyl groups without significant yield loss. Use 3'-CPR II CPG (20-2903) instead.
Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
Deprotection: Cleavage of the oligonucleotide from this support requires 2 hours at room temperature with ammonium hydroxide. Complete the deprotection using the protocol required by the nucleobases. Note: complete deprotection to the 3'-phosphate requires 17 hr @ RT or 4 hr @ 55°C in ammonium hydroxide, or 8 hr @ RT in K2CO3/MeOH Technical Bulletin
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: Not Applicable

CHEMICAL PHOSPHORYLATION

Chemical Phosphorylation Reagent is most commonly used to phosphorylate the 5’-terminus of an oligonucleotide. Although this product is also successful in 3’-phosphorylation, 3’-Phosphate CPG allows direct preparation of oligonucleotides with a 3’-phosphate group. Chemical Phosphorylation Reagent II contains a DMT group on a side chain which is stable to base cleavage and can be left on the oligonucleotide for use in RP purification. The DMT group is later removed with aqueous acid and the side chain is eliminated after brief treatment with aqueous ammonium hydroxide to yield the 5’-phosphate.1 Solid CPR II is similar in performance to CPR II but it is easier to prepare aliquots since it is a powder. Many researchers treat synthesis supports with a hindered base (e.g., diethylamine, diisopropylethylamine, or DBU) post-synthesis to eliminate and remove the cyanoethyl phosphate groups. In this way, the acrylonitrile formed in situ is removed from the support and is not available to alkylate dT residues at the N3 position in the oligos. Since the sulfonylethyl group in 3’-Phosphate CPG is also susceptible to ß-elimination leading to oligo cleavage, this technique is not compatible with 3’-phosphate CPG. Using CPR II CPG, which is base labile but does not support ß-elimination, the cyanoethyl groups can be removed from the oligo prior to cleavage and base deprotection. ABI-style vials and columns are supplied unless otherwise requested.

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3'-Phosphate CPG

3'-Phosphate CPG

Glen Research