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Oligo Synthesis

Oligo Synthesis : Supports,CPGs

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5-Me-dC-CPG

5-Me-dC-CPG

Glen Research

Catalogue No.DescriptionPack SizePriceQty
20-2060-015-Me-dC-CPG 0.1g £38.00£34.96Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2160-415-Me-dC-CPG 4x1.0µm £152.00£139.84Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
20-2160-425-Me-dC-CPG 4x0.2µm £91.00£83.72Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

5-Me-dC-CPG

5-Me-dC-CPG

Glen Research

5-Me-dC-CPG

Structure

Catalog Number: 20-2060-xx

(20-2160-xx)

Description: 5-Me-dC-CPG

5'-Dimethoxytrityl-N-benzoyl-5-methyl-2'-deoxyCytidine,
2'-succinoyl- long chain alkylamino-CPG


F.W.: 303.21

Diluent: Not Applicable
Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
Deprotection: Deprotect using the protocol required by the nucleobases.
Storage: Controlled room temperature or lower, dry
Stability in Solution: Not Applicable

bases affecting duplex stability

C-5 methyl pyrimidine nucleosides are known to stabilize duplexes relative to the non-methylated bases. Therefore, enhanced binding can be achieved using 5-methyl-dC in place of dC, duplex melting temperature being increased by 1.3°. Improved stacking in this case is believed to be brought about by elimination of water molecules from the duplex. 2,6-Diaminopurine 2'-deoxyriboside (2-amino-dA) forms an additional hydrogen bond with Thymidine, thereby leading to duplex stabilization with a melting temperature increase of 3°. Our 2-amino-dA monomer exhibits fast and effective deprotection in ammonium hydroxide and it is stabilized to depurination during synthesis. Sequences with high GC content may contain mismatches and still hybridize because of the high stability of the G-C base pair. The N4-ethyl analogue of dC (N4-Et-dC) hybridizes specifically to natural dG but the stability of the base pair is reduced to about the level of an AT base pair.

AP-dC (G-clamp) enhances oligo hybridization since the AP-C....G base pair contains 4 hydrogen bonds, which makes the interaction much stronger than the regular C....G base pair with its 3 hydrogen bonds.

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5-Me-dC-CPG

5-Me-dC-CPG

Glen Research

MSDS

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200