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In Vitro Toxicology

In Vitro Toxicology: Mutagenicity kits

The Ames II / Ames MPF Microfluctuation Assays are all modifications of the traditional Ames plate incorporation assay.

The Ames II assay uses the S. typhimurium strains TA98 and TAMix (a mixture of 6 strains, each detecting a specific base-pair substitution), whereas the Ames MPF® kits are available with the strains TA98, TA100, TA1535, TA1537, E.coli WP2 uvrA or E.coli WP2 pKM101.

 

Benefits of Xenometrix Ames Mutagenicity Assays over traditional Ames Procedures

  • Easy, compact and high throughput liquid format with 384 well plates.

  • Simple detection method with easy "readout" (as compared to the traditional assay)

  • Kits include all five types strains cited in OECD 471 (CoA supplied), quality controlled according to the same guideline 

  • Much less  test compound required.  (10 mg per strain if tested in triplicates, +/- S9, 6 semi-logarithmic compound dilutions)

  • Ames MPF is the preferred system for genotoxic impurities due to its low consumption of compound

  • Significantly less consumption of S9

  • Less environmental pollution due to significantly less "contaminated" waste

  • Less user intervention

  • Download presentation 

Ames MPF Penta 1 Test Kits - semi-solid (98/100/1535/1537/ECWP2)

Complete set of tester strains

Xenometrix AG

Catalogue No.DescriptionPack SizePriceQty
XC01-512-S1-PAmes MPF PENTA I - 1 Sample Kit - S9 - Pos.Con.1 sample kit £1,385.00 Quantity Add to Order
XC01-512-S2-PAmes MPF Penta1 - 10 Sample Kit rat liver S9, Phenobarbital/ß-Naphtoflavone induced Pos. Control 1 test £1,380.00 Quantity Add to Order
XC10-512Ames MPF Penta1 I - 10 Sample Kit - 480 Measuring Points / Strain10 sample kit £4,768.00 Quantity Add to Order
XC10-512-S1-PAmes MPF Penta I - 10 Sample Kit - S9 - Pos.Con10 sample kit £5,338.00 Quantity Add to Order
XC10-512-S2-PAmes MPF Penta1 - 10 Sample Kit rat liver S9, Phenobarbital/ß-Naphtoflavone induced Pos. Control 10 tests £5,628.00 Quantity Add to Order

Description

The Ames MPF Penta I assay comprises the histidine auxotrophic Salmonella tester strains TA98, TA100, TA1535, TA1537 and two tryptophan auxotrophic tester strains E.coli wp2 (E.coli wp2 [pKM101] and E.coli wp2 uvrA, combined exposure) as well as all media required to perform the assay. Kits including additional S9 and Positive Controls are also available.

The strains included in this kit meet the requirements of the OECD guideline 471 for testing chemicals.

Ames MPF Penta 1 - 1 Sample Kit

Kit Contents

(depending on kit size)

1 or 10 Vials semisolid TA98
1 or 10 Vials semisolid TA100
1 or 10 Vials semisolid TA1535
1 or 10 Vials semisolid TA1537
1 or 10 Vials semisolid E.coli uvrA and E.coli pKM101
1 or 10 Vials Ampicillin
Growth -, Exposure - and Reversion Indicator Media
All media and strains provided are sufficient to analyze at least  1 or 10 compounds,
triplicates, 6 concentrations, negative and positive controls, /- S9

 

10 sample preps

5 x 480 Measuring Points: 10 Samples per Strain if tested in Triplicates, 6 Dilutions, /- S9, Negative and Positive Controls

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200