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In Vitro Toxicology

In Vitro Toxicology: Mutagenicity kits

The Ames II / Ames MPF Microfluctuation Assays are all modifications of the traditional Ames plate incorporation assay.

The Ames II assay uses the S. typhimurium strains TA98 and TAMix (a mixture of 6 strains, each detecting a specific base-pair substitution), whereas the Ames MPF® kits are available with the strains TA98, TA100, TA1535, TA1537, E.coli WP2 uvrA or E.coli WP2 pKM101.

 

Benefits of Xenometrix Ames Mutagenicity Assays over traditional Ames Procedures

  • Easy, compact and high throughput liquid format with 384 well plates.

  • Simple detection method with easy "readout" (as compared to the traditional assay)

  • Kits include all five types strains cited in OECD 471 (CoA supplied), quality controlled according to the same guideline 

  • Much less  test compound required.  (10 mg per strain if tested in triplicates, +/- S9, 6 semi-logarithmic compound dilutions)

  • Ames MPF is the preferred system for genotoxic impurities due to its low consumption of compound

  • Significantly less consumption of S9

  • Less environmental pollution due to significantly less "contaminated" waste

  • Less user intervention

  • Download presentation 

Ames MPF E.coli pKM Test Kits - semi-solid

The Ames MPF E.coli pKM101 Assay comprises the tryptophan auxotrophic tester strain E.coli wp2 [pKM101] for detecting AT base-pair mutations at the primary reversion site and all media required to perform the assay

Xenometrix AG

Catalogue No.DescriptionPack SizePriceQty
XC01-116Ames MPF E.coli pKM - 1 Sample Kit - 48 Measuring Points1 sample kit POA Quantity Add to Order
XC01-116-S2-Plyophilized rat liver S9, Phenobarbital/ß-Naphtoflavone induced Pos. Control 1 test POA Quantity Add to Order
XC10-116Ames MPF E.coli pKM - 10 Sample Kit - 480 Measuring Points10 sample kit POA Quantity Add to Order
XC10-116-S2-Plyophilized rat liver S9, Phenobarbital/ß-Naphtoflavone induced Pos. Control 10 Tests POA Quantity Add to Order

Description

The Ames MPF E.coli pKM101 Assay comprises the tryptophan auxotrophic tester strain E.coli wp2 [pKM101] for detecting AT base-pair mutations at the primary reversion site and all media required to perform the assay. Kits including additional S9 and Positive Controls are also available. E.coli wp2 strains detect certain oxidizing mutagens, cross-linking agents and hydrazines.

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