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Lab Reagents

Lab Reagents: Absolute Lectins

Absolute Lectins, perfect for Glycoscience Medicago is a primary manufacturer of a wide variety of exceptionally pure lectins purified by affinity chromatography. Lectins are non-enzymatic proteins of non-immunoglobulin origin that bind specifically and reversibly to carbohydrate moieties without altering the covalent structure of the glycosyl ligands. The term ‘lectin’ is derived from the latin word legere, meaning ‘to select’. The specificity of a lectin is usually defined by the monosaccharides or oligosaccharides that are best at inhibiting the agglutination or precipitation caused by the lectin. Lectins are usually of plant origin but do occur in many types of organism; some are glycoproteins and may be soluble or membrane-bound.

The biological function of lectins is far from fully explored. Their specificity enables binding to glycoproteins and polysaccharides as well as agglutination of erythrocytes and stimulation of blood lymphocytes. Because of their ability to distinguish glycosyl ligands on human red blood cells, lectins can be used for blood typing.Immobilized lectins can be used in affinity chromatography to purify and isolate glycoproteins, glycolipids, polysaccharides, viruses and cells.

Carbohydrate-containing substances bound to the lectin may be eluted with a competitive binding substance. Medicago offers lectins as a freeze-dried powder or as a solution manufactured under aseptic conditions. If you don’t find the lectin you are looking for, we can certainly produce it for you. Furthermore, we offer different custom lectin conjugates on request.

•Ultrapure quality

•High activity

•Wide range

•Lyophilized powder

•Custom lectins and conjugates

Arachis hypogaea lectin (PNA)

Arachis hypogaea lectin or Peanut Agglutinin (PNA) is isolated from peanuts and purified by affinity chromatography. The lectin has a molecular weight of 110 kDa and consists of four identical subunits of approximately 27 kDa each

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
MC-05-0116-10Arachis hypogaea lectin (PNA) 10 mg £130.00 Quantity Add to Order
MC-05-0116-50Arachis hypogaea lectin (PNA) 50 mg £285.00 Quantity Add to Order

Description

Product Description

Arachis hypogaea lectin or Peanut Agglutinin (PNA) is isolated from peanuts and purified by affinity chromatography. The lectin has a molecular weight of 110 kDa and consists of four identical subunits of approximately 27 kDa each (1).

PNA is a carbohydrate-free protein that displays specificity towards ß-D-Gal(1-3)-D-galNAc (3). It has potent anti-T activity and can be used to distinguish between human lymphocyte subsets. PNA has been used in tumour tissue determination for transitional mucosa malignancies. The lectin also agglutinates neuraminidase-treated human erythrocytes at < 0.1 µg/ml after trypsin treatment of cells and its activity is inhibited by lactose and galactose (1).

 

Medicago’s PNA lectin is provided as a white to light yellow lyophilized powder from a buffer containing 10 mM NH4HCO3. The purity is determined by SDS-PAGE, which generates one band at 25-27 kDa. The lectin is available in vials containing 50 mg or 10 mg lyophilized powder and the product is to be used for laboratory work only.


Features 

  • Ultrapure quality
  • Strong anti-T activity
  • Sugar specificity: ß-D-Gal-(1-3)-D-GalNAc
  • Agglutinates rabbit erythrocytes at < 0.1 µg/ml after trypsin treatment of the cells 
  • Lyophilized powder

Directions for use

The lectin may be reconstituted with 2 ml of sterile PBS buffer, pH 7.4 before use. Spin the vial gently until full dissolution. Aggregation is thought to occur in the presence of high concentrations of 2-mercaptoethanol.

 

Shipping and storage

The product is shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than three years from production date when stored below -20°C. After reconstitution with deionized water, the solution may be stored frozen in working aliquots for up to 12 months.

 

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

References

References

(1) The purification, composition, and specificity of the anti-T lectin from peanut (Arachis hypogaea). R Lotan, E Skutelsky, D Danon and N Sharon. JBiol. Chem Vol. 250, No. 21

(2) Conformation, protein-carbohydrate interactions and a novel subunit association in the refined structure of peanut lectin-lactose complex.

Banerjee, R., Das, K., Ravishankar, R., Suguna, K., Surolia, A., Vijayan, M. (1996) J.Mol.Biol. 259: 281–96.

(3) Liener I. E., Sharon N., Goldstein I. J., (1986) The Lectins – Properties, Functions and Applications in Biology and Medicine.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Notes

Specifications

Appearance: White to light yellow lyophilized powder

Source: Peanuts

Activity: Agglutinates rabbit erythrocytes at a concentration of < 0.1 μg/ml after trypsin treatment. Agglutination is inhibited by 10 mM D-galactose.

Microorganisms: < 100 CFU/g                                                              

Protein: > 95 %                                                                                                                            

Identity: SDS-PAGE, one band at 25-27 kDa                              

Shelf life: > Three years when stored at -20°C

Specifications

Appearance: White to light yellow lyophilized powder

Source: Peanuts

Activity: Agglutinates rabbit erythrocytes at a concentration of < 0.1 μg/ml after trypsin treatment. Agglutination is inhibited by 10 mM D-galactose.

Microorganisms: < 100 CFU/g                                                              

Protein: > 95 %                                                                                                                            

Identity: SDS-PAGE, one band at 25-27 kDa                              

Shelf life: > Three years when stored at -20°C

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Applications & Benefits

Applications

  • Probe in histochemistry and immuno-histochemistry
  • Human erythrocyte/lymphocyte studies

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200