Product description
3, 3’, 5, 5’-Tetramethylbenzidine (TMB) substrate is a ready-to-use liquid substrate for HRP, specially developed for ELISA procedures. EC-Blue Enhanced™ Substrate turns deep blue (620-650 nm) in the presence of a peroxidase-labelled conjugate. Colour development can be stopped by adding an equal volume of 0.5 M H2SO4 (Article no. 10-9500-50), which turns the substrate yellow (450 nm).
One bottle of EC Blue consists of a solution containing proprietary ready-mixed chromogen substrate reagents, pH 3.8. The solution has a straw-yellow colour at room temperature and a light greenish tint when stored at 4°C. The colour returns to normal when warmed overnight to room temperature.
Features
- Ready-to-use liquid substrate
- Substrate for assays using horseradish peroxidase (HRP) conjugate
- Low background
- High sensitivity
- Long stability
For laboratory use only.
Storage and stability
Store at 2°C to 8°C. Do not freeze and do not expose to direct sunlight. Prolonged exposure to light is not recommended. Store in an amber bottle. As some metal ions can oxidize TMB, allow only glass or plastic to come in contact with the substrate.
Never pipette directly from the bottle. Do not leave the cap off. EC-Blue Enhanced™ Reagent is stable for at least 18 months when stored at 2°C to 8°C.
Directions for use
No additional mixing reagents are required. The material is not dependent on room temperature.
Shipping and Storage
The product is stable for 12 months if stored at 2°c to 8°C.
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