Applications

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Extraction & Purification - DNA: Total DNA

Fast and easy methods for the micro scale purification of total (genomic and mitochondrial) DNA from various biological samples.

Proteinase K

Proteinase K is a proteolytic enzyme. The enzyme uses Ca2+ ions for structural stability. Due to the high total activity of the enzyme, lysis can be carried out in the presence of EDTA. Proteinase K is therefore excellently useful for the extraction of nucleic acids from various biological samples.

Minerva Biolabs

Catalogue No.	Description	Pack Size	Price	Qty	Change to: €
56-0002	Proteinase K	50 extractions	£26.00	Quantity	Add to Order

Description

Recommended Use / Scope

Minerva Biolabs DNA Extraction Kits are used for the extraction of DNA from ordinary samples, like cell culture supernatants containing up to 10⁶ cells, with high efficiency. Anyhow, other samples matrices may contain protein with a concentration above 10 mg/ml, like cell pellets, serum, or fermentation products. These samples may require an additional Proteinase K treatment to increase the overall DNA yield for highest sensitivity.

In combination with Minerva Biolabs´ DNA Extraction kits 10 µl of the Proteinase K solution is used for each sample after mixing the sample with Conditioner (MB DNA Extraction Kit) or Buffer A (Microsart AMP Extraction).

Quality Control

All lots of this product are routinely checked by the extraction of 10 CFU/ml of mycoplasma from a defined complex sample matrix in comparison to an untreated sample. The extracts were used as template for mycoplasma PCR with the Intego® Mycoplasma Kit for qPCR using a protocol for 10 µl sample volume.

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Notes

The freeze-dried product shall be stored at 2 to 8 °C. The rehydrated stock solution must be stored at < -18 °C. Avoid repeated freezing and thawing. Preparation of aliquots might be required if more than 5 freezing/thawing cycles are required.