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Oligo Synthesis

Oligo Synthesis : CEPs

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N6-Methyl-A-CE Phosphoramidite

5'-Dimethoxytrityl-N-methyl-N-phenoxyacetyl-Adenosine,2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-3005-02N6-Methyl-A-CE Phosphoramidite0.25g £1,386.00 Quantity Add to Order
10-3005-90N6-Methyl-A-CE Phosphoramidite100umole £582.00 Quantity Add to Order
10-3005-95N6-Methyl-A-CE Phosphoramidite50umole £300.00 Quantity Add to Order

Description

Formula: C55H70N7O9PSi

M.W.: 1032.25

F.W.: 343.23

 

Diluent: Anhydrous Acetonitrile

Coupling: 12 minute coupling time is recommended. To avoid any exchange of the iPr-Pac group on the G with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).

Deprotection: RNA Deprotection-see bulletin Technical Bulletin

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 1-2 days

 

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Protocols

MSDS

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References

Glen Report 26.21: Reversible m6A RNA modification
Glen Report 26.22: New Product - N6-Me-A - Reversible m6A RNA modification

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Notes

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack Size Grams/ Pack 0.1M Dil. (mL) LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-3005-02 0.25grams .25grams 2.42 67.33 40.4 25.25 18.36 13.47 3.37
10-3005-90 100µmoles .103grams 1 20 12 7.5 5.45 4 1
10-3005-95 50µmoles .052grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack Size Grams/ Pack Dilution (mL) Molarity 50nm 0.2µm 1µm 15µm  
Approximate Number of Additions
10-3005-02 0.25grams .25grams 3.61 .07 N/A N/A 13.71 N/A  
10-3005-90 100µmoles .103grams 1.5 .07 N/A N/A 4.92 N/A  
10-3005-95 50µmoles .052grams .75 .07 N/A N/A 1.79 N/A  
Mermade
Cat.No. Pack Size Grams/ Pack Dilution (mL) Molarity 30nm 200nm 1000nm    
Approximate Number of Additions
10-3005-02 0.25grams .25grams 3.61 .07 N/A 10.87 10.21    
10-3005-90 100µmoles .103grams 1.5 .07 N/A 4.06 3.82    
10-3005-95 50µmoles .052grams .75 .07 N/A 1.65 1.55

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