Cambio - Excellence in Molecular Biology

Oligo Synthesis

Oligo Synthesis : Reagents, Labelling

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Methylene Blue NHS Ester

Methylene Blue NHS Ester 3-(N,N-dimethylamino)-7-[N-(3-(N-succinimidyl)-carboxyethyl)- N-(methyl)-amino]-phenothiazin-5-ium perchlorate

Glen Research

Catalogue No.DescriptionPack SizePriceQty
50-1960-23Methylene Blue NHS Ester5.4mg £614.00 Quantity Add to Order


Glen Report 26.24: DNA-Mediated Charge Transport: A novel property of DNA with diverse applications
Glen Report 26.25: New Product - Methylene Blue NHS Ester

Diluent: Acetonitrile, DMSO, DMF

Labeling: For labeling 0.2µmoles of an amino-modified oligonucleotide, dissolve 5.4mg of the Methylene Blue NHS Ester in 60µL of DMSO (Acetonitrile or DMF are also acceptable). Dissolve the amino-modified oligonucleotide in 500µL 0.1 M Carbonate/Bicarbonate buffer (pH 9). Add 12µL of the Methylene Blue NHS Ester solution and vortex. Let react for 2 hours at room temperature and desalt.

Storage: Freezer storage, -10 to -30°C, dry

Formula: C22H23N4O8SCl 
M.W.: 538.96 
F.W.: 425.89 




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