Cambio IDA MagBeads
The His tag is the most widely used affinity tag due to its small size and versatility under native and denaturing conditions, as well as in presence of detergents and many other additives. Magnetic beads are ideal for protein purification from dilute supernatants and for pull-down experiments. The agarose surface of our MagBeads is identical to that of Cambio Agarose, making them an ideal combination for small-scale screening and scaling up.
Cambio IDA MagBeads are ferrimagnetic agarose beads coupled to an IDA chelating ligand. IDA MagBeads can be loaded with transition metals to obtain different specificities for his-tagged proteins, or for other applications such as purification of phosphorylated proteins. Cambio IDA MagBeads are delivered as a 25% suspension.
Why Cambio IDA MagBeads?
- Can be loaded with most transition metals
- Low unspecific binding
- Stable under commonly used concentrations of DTT and EDTA
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