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Cell Information:
Human Osteoblasts (HOb) sythesize bone matrix. Matrix mineralization can be induced in the presence of ascorbic acid and B-Glycerophosphate. Hob are isolated from normal human bone. These cells are commonly used for micro- and macroscopic investigations of the skeletal system, such as the effects of inflammatory mediators, the effects of bone morphogenic proteins, the physiological control of bone remodeling, the regulation of bone metabolism, and the study of the chemicals associated with physical trauma.
Applications:
- In vitro bone models
- Mineralization
- Osteoporosis
- Gene expression
- Hormone treatments
- Osteopenia
- Paget's Disease
The Transfection Reagent:
Transfection of primary cells, such as HOb, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.
Human Osteoblast (HOb, HOb-OA, HOb-RA, NHOst) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on HOb. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HOb. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Osteoblast (HOb, HOb-OA, HOb-RA, NHOst) Avalanche™ Transfection Reagent for transfection on HOb. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
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Highest efficiency to ensure experimental success
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Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
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Simplest and straightforward protocols
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Compatible with serum
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Suitable for Reverse Transfection
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Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
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Economical: High efficiency means less amount of nucleic acid & reagent is needed
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Developed and manufactured by EZ Biosystems
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Human Osteoblast (HOb, HOb-OA, HOb-RA, NHOst) Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies.
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |