Transfection-EZ Biosystems: Primary Cells
Avalanche®- primary cell type-specific Transfection Reagent series?
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines). Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
If you work on cell lines rather than primary cells then click HERE
Human Epidermal Keratinocyte (HEK, NHEK) Avalanche™ Transfection Reagent
ransfection of primary cells, such as HEK, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.
Human Epidermal Keratinocyte (HEK, NHEK) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on HEK. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HEK. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Epidermal Keratinocyte (HEK, NHEK) Avalanche™ Transfection Reagent for transfection on HEK
EZ Biosystems
Description
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Cell Information:
Human Epidermal Keratinocytes (HEK) are primary cells isolated from normal human neonatal foreskin or adult skin. HEK is a suitable in vitro model for dermal toxicology tests in screening new skin products, and for developing dermal skin substitutes. HEK has been used in the following applications:
- Epidermal model generation
- Skin cancer
- Toxicity (skin absorption studies)
- Differentiation models (Ca free medium needed)
- Epidermal disease research
- Dermatitis/eczema, psoriasis, sebaceous cysts, folliculitis, and impetigo
- Dermal burns
- Wound healing
The Transfection Reagent:
Transfection of primary cells, such as HEK, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.
Human Epidermal Keratinocyte (HEK, NHEK) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on HEK. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HEK. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Epidermal Keratinocyte (HEK, NHEK) Avalanche™ Transfection Reagent for transfection on HEK. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Specifically optimized to deliver nucleic acids into HEK
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
- Suitable for Reverse Transfection
- Compatible with transfection in any plate formats
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Developed and manufactured by EZ Biosystems
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| Data: |
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| FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human epidermal keratinocytes after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human epidermal keratinocytes. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Epidermal Keratinocyte Avalanche Transfection Reagent. |
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For Other Cells: |
Human Epidermal Keratinocyte (HEK, NHEK) Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies.
LNCaP Cell
DU 145 Cell
22Rv1 Cell
VCaP Cell
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200