Transfection-EZ Biosystems: Primary Cells
Avalanche®- primary cell type-specific Transfection Reagent series?
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines). Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
If you work on cell lines rather than primary cells then click HERE
Human Bladder Epithelial Cell (HBlEpC) Avalanche™ Transfection Reagent
Transfection of primary cells, such as HBlEpC, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.
Human Bladder Epithelial Cell (HBlEpC) Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on HBlEpC. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HBlEpC. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Bladder Epithelial Cell (HBlEpC) Avalanche® Transfection Reagent for transfection on HBlEpC
EZ Biosystems
Description
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Cell Information:
Beyond the role of the bladder epithelium in forming a barrier, this tissue may play an unappreciated but important role in water, salt, and solute homeostasis. One of the most exciting functions of the bladder epithelium is its potential role as a sensory transducer.
Urothelial carcinoma of the bladder is unique among epithelial carcinomas in its divergent pathways of tumorigenesis. Low-grade papillary tumors rarely become muscle-invasive and they frequently harbour gene mutations that constitutively activate the receptor tyrosine kinase–Ras pathway. By contrast, most high-grade invasive tumors progress to life-threatening metastases and have defects in the p53 and the retinoblastoma protein pathways.
Human bladder epithelial cells (HBlEpC) can be used as a powerful tool in studying not only bladder epithelial development and function, but also the processes by which epithelial cells achieve and maintain their specialized plasma membrane domains, and how these functions are disrupted in disease.
The Transfection Reagent:
Transfection of primary cells, such as HBlEpC, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.
Human Bladder Epithelial Cell (HBlEpC) Avalanche® Transfection Reagent is a new, proprietary solution specifically designed for transfection on HBlEpC. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HBlEpC. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Bladder Epithelial Cell (HBlEpC) Avalanche® Transfection Reagent for transfection on HBlEpC. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
Features:
- Specifically optimized to deliver nucleic acids into HBlEpC
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Deliver single or multiple plasmids
- synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Simplest and straightforward protocols
- Compatible with serum
- Compatible with transfection in any plate formats
- Developed and manufactured by EZ Biosystems
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| Data: |
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| FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human renal epithelial cell (HRE) after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human renal epithelial cell (HRE). This test result was confirmed with repeat experiments. The candidate formulas were also tested in human bladder epithelial cell (HBlEpC) by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as Human Bladder Epithelial Cell (HBlEpC) Avalanche Transfection Reagent. |
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For Other Cells: |
Human Bladder Epithelial Cell (HBlEpC) Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies.
Human Urethra Epithelial Cell (HUEpC)
Human Renal Epithelial cell (HRE)
Human Prostate Epithelial Cell (HPrEpC)
Human Cervical Epithelial Cell (HCvEpC)
Human Placental Epithelial Cell (HPlEpC)
Human Mammary Epithelial Cell (HMEpC, HMEC)
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list |
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200