The AmpliCap™ SP6 High Yield Message Maker Kit produces capped RNA by in vitro transcription using SP6 RNA polymerase and the standard cap analog m7G[5']ppp[5']G. A 2 hour, 20 μl AmpliCap reaction yields up to 35 μg of m7G-capped RNA from 1 μg of the 1.4 kb standard SP6 control template DNA.
The m7G[5']ppp[5']G cap analog/NTP PreMix contains all four ribonucleotides and the monomethylated cap analog. The PreMix ensures the optimal concentration of each NTP and ratio of methylated cap analog to GTP (4:1), maximizing transcript capping (~80%) and yield. Because the concentration of GTP in the reaction is limiting, the cap analog is preferentially incorporated as the first or 5'-terminal G of the transcript.
CELLSRIPT also offers the AmpliCap-Max™ T7 High Yield Message Maker Kit and the MessageMAX™ T7 ARCA-Capped Message Transcription Kit, both of which produce unsurpassed yields of capped in vitro transcripts (up to 60 μg of RNA in a 30 minute standard control reaction with an 80% capping efficiency). The AmpliCap-Max Kit uses the standard RNA Cap Analog m7G[5']ppp[5']G and the MessageMAX Kit uses the anti-reverse cap analog m27,3'-OG[5']ppp[5'].
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