The AmpliCap-Max™ T7 High Yield Message Maker Kit produces capped RNA by in vitro transcription using T7 RNA polymerase and the standard cap analog m7G[5']ppp[5']G. A 30 minute, 20 μl AmpliCap-Max reaction yields up to 60 μg of m7G-capped RNA from 1 μg of the 1.4 kb standard T7 control template DNA.
The m7G[5']ppp[5']G cap analog/NTP PreMix contains all four ribonucleotides and the monomethylated cap analog. The PreMix ensures the optimal concentration of each NTP and ratio of methylated cap analog to GTP (4:1), maximizing transcript capping (~80%) and yield. Because the concentration of GTP in the reaction is limiting, the cap analog is preferentially incorporated as the first or 5'-terminal G of the transcript.
CELLSCRIPT also offers the MessageMAX™ T7 ARCA-Capped Message Transcription Kit which also produces up to 60 μg of capped RNA in a 30 minute standard control reaction with an 80% capping efficiency. The MessageMAX Kit uses the anti-reverse cap analog (ARCA), m27,3'-OG[5']ppp[5'].
If you cannot find the answer to your problem below then please
contact us or telephone 01954 210 200