Cell Information:
In addition to functioning as physical barriers against microbes, the urethral epithelial linings participate in the first line of host defense by production of a variety of proinflammatory mediators when exposed to microbes and microbial agents. This might occur via adhesion-mediated signalling by receptor interaction or via attachment-facilitated presentation of conserved microbial patterns to TLRs on epithelial cells.
Primary Human Urethra Epithelial Cell (HUEpC) is a good in vitro model for pathogenesis studies of urethral epithelial cells in various diseases.
The Transfection Reagent:
Transfection of primary cells, such as HUEpC, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.
Human Urethra Epithelial Cell (HUEpC) Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting HUEpC. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HUEpC. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series
Features:
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Unique Formulation-Maximize transfection performance in HUEpC
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Extremely gentle to cells
- Single or multiple plasmids transfection
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Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines.
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Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells.
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Compatible with serum
- Developed and manufactured by EZ Biosystems
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| Data: |
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| FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human renal epithelial cell (HRE) after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human renal epithelial cell (HRE). This test result was confirmed with repeat experiments. The candidate formulas were also tested in human urethra epithelial cell (HUEpC) by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as Human Urethra Epithelial Cell (HUEpC) Avalanche Transfection Reagent. |
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For Other Cells: |
Human Urethra Epithelial Cell (HUEpC) Avalanche® Transfection Reagent can also be used on the following cells with high transfection efficiencies.
Human Prostate Epithelial Cell (HPrEpC)
Human Cervical Epithelial Cell (HCvEpC)
Human Bladder Epithelial Cell (HBlEpC)
Human Placental Epithelial Cell (HPlEpC)
Human Mammary Epithelial Cell (HMEpC, HMEC)
Human Airway Epithelial Cell (HBEpC, HTEpC, NHBE, SAEC)
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
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