Catalog Number:EZT-LL20-1
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| Cell Synonyms:LLC1, CRL-1642 |
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Cell Line Information:
Designations: LL/2
Organism: Mus musculus (mouse)
Strain: C57BL
Tissue: Lewis lung carcinoma; lung
Growth Properties: mixed: adherent and suspension
Depositors: J.S. Bertram
Lewis lung carcinoma is a cell line established from the lung of a C57BL mouse bearing a tumor resulting from an implantation of primary Lewis lung carcinoma.
The cells are resistant to 1,3-bis-(2-chloroethyl)-1-nitrosourea, but are sensitive to methotrexate. The cells are reported to be highly tumorigenic, in C57BL mice but weakly metastatic.
The Transfection Reagent:
Transfection of suspension cells, or suspension/adherent mixed cells, such as LL/2 Cells, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect suspension cells, as well as suspension/adherent mixed cells.
LL/2 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on LL/2 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for LL/2 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of LL/2 Cell Avalanche™ Transfection Reagent for transfection on LL/2 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Specifically optimized to deliver nucleic acids into LL/2 Cells
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Animal-free
- Simplest and straightforward protocols
- Compatible with serum
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
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| Data: |
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| FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on LL/2 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for LL/2 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this LL/2 Cell Avalanche Transfection Reagent. |
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For Other Cells: |
LL/2 Cell Avalanche™ Transfection Reagent (mouse lung carcinoma cell) can also be used on the following cells with high transfection efficiencies.
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
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