Cell Line Information:
Designations: AsPC-1
Organism: Homo sapiens (human)
Tissue: adenocarcinoma; pancreas; derived from metastatic site: ascites
Age: 62 years
Gender: female
Ethnicity: Caucasian
Morphology: epithelial
Products: carcinoembryonic antigen (CEA); human pancreas associated antigen; human pancreas specific antigen; mucin
Growth Properties: adherent
Depositors: M.H. Tan
The line was derived from nude mouse xenografts initiated with cells from the ascites of a patient with cancer of the pancreas.
The Transfection Reagent:
AsPC-1 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting AsPC-1 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for AsPC-1 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Unique formulation-maximize transfection performance in AsPC-1 Cells.
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Simplest and straightforward protocols
- Compatible with serum
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Developed and manufactured by EZ Biosystems
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| Data: |
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| FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on Capan-1 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for Capan-1 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on AsPC-1 cells by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as AsPC-1 Cell Avalanche Transfection Reagent. |
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For Other Cells: |
AsPC-1 Cell Avalanche™ Transfection Reagent (human pancreas adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies.
AR42J Cell
Capan-1 Cell
PANC-1 Cell
BxPC-3 Cell
293 Cell
293T/17 Cell
SK-OV-3 Cell
U-2 OS cell
Saos-2 Cell
SK-N-SH Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200