Cell Line Information:
Depositors: R Cailleau
Growth Properties: adherent
Organism: Homo sapiens
Source: mammary gland; breast
Derived from metastatic site: pleural effusion
Cell Type: epithelial
Applications: transfection host
Receptors: epidermal growth factor (EGF), Transforming growth factor alpha (TGF alpha)
Age: 51 years adult
Comments: The cells express the WNT7B oncogene [PubMed: 8168088].
MDA-MB-231 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.
The Transfection Reagent:
MDA-MB-231 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting MDA-MB-231 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for MDA-MB-231 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
- Specifically optimized to deliver nucleic acids into MDA-MB-231 Cells
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Efficient for plasmid DNA/siRNA co-transfection
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Simplest and straightforward protocols
- Compatible with serum
- Suitable for Reverse Transfection
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
|FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on MDA-MB-453 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for MDA-MB-453 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on MDA-MB-231 cells by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as MDA-MB-231 Cell Avalanche Transfection Reagent.
|FIG. 2. MDA-MB-231 cells were transfected with GFP vector (pEGFP-N3) by using MDA-MB-231 Cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 48 hours post transfection.
For Other Cells:
|MDA-MB-231 Cell Avalanche™ Transfection Reagent (human breast carcinoma cell) can also be used on the following cells with high transfection efficiencies.
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200