Cambio - Excellence in Molecular Biology

Extraction & Purification - DNA

Extraction & Purification - DNA: Fragment DNA & PCR clean-up

Fragment DNA purification, Post PCR cleanup, Gel purification

Expin Gel SV

DNA extraction from agarose gel is a common technique for isolation of specific fragments from reaction mixtures. However, the conventional methods either fail to completely remove agarose, shear the DNA or result in low yields. Expin Gel SV kit takes advantage of glass fibre ....

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Description

 

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DNA extraction from agarose gel is a common technique for isolation of specific fragments from reaction mixtures. However, the conventional methods either fail to completely remove agarose, shear the DNA or result in low yields. ExpinTM Gel SV kit takes advantage of glass fiber membrane to recover DNA of 80 bp to 10 kb from most grades of agarose gel in yields reaching 90 %.

 

Features and Benefits

¦ Spin or vacuum column format
¦ DNA extraction from standard and low-melting agarose (TAE, TBE)
¦ Stable and consistent result
¦ Rapid and convenient procedure
¦ Instant use : No need of additional materials
¦ High purity : 1.8 ~ 2.0
¦ Yield : 70 ~ 85 % average (80 bp ~ 10 kb)
¦ No use of organic solvents
¦ Complete removal of ethidium bromide
¦ pH indicator in binding buffer
¦ Ready for ligation, sequencing, labeling, PCR, enzyme assay and etc.

 

 Procedure

      

 

 Specification

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Protocols

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References

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If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200