Cambio - Excellence in Molecular Biology

Extraction & Purification - DNA

Extraction & Purification - DNA: Clinical

Isolate and purify DNA from clinical materials, such as buccal swabs, blood, saliva tissues and stool samples.

Exgene™ Blood SV

Exgene™ Blood SV kit provides a simple and rapid method for the isolation of total DNA from fresh or frozen whole blood, buffy coat, serum, plasma, virus and cultured cells. Purification procedure is so simple andoptimized to simultaneous processing of multiple samples. Exgene™ Blood SV yields pure DNA ready for direct PCR in just 20 minutes (mini) and 1 hour (Midi / MAXI). There is no need phenol extraction or alcohol precipitation.

GeneAll

Description

ExgeneTM Blood SV kit provides a simple and rapid method for the isolation of total DNA from fresh or frozen whole blood, buffy coat, serum, plasma, virus and cultured cells. Purification procedure is so simple and
optimized to simultaneous processing of multiple samples. ExgeneTM Blood SV yields pure DNA ready for direct PCR in just 20 minutes (mini) and 1 hour (Midi / MAXI). There is no need phenol extraction or alcohol precipitation.

Features and Benefits

¦ Spin or vacuum column format
¦ Accurate and consistent DNA extraction from whole blood, buffy coat, serum, plasma,cultured cells
¦ Instant use : No need of additional materials
¦ Fast, safe and simple procedure completed in 20 minutes (mini), 1 hour (Midi, MAXI)
¦ High purity : 1.8 ~ 2.0
¦ No use of organic solvents
¦ Ready for use in PCR, southern blotting and other enzymatic reactions

Protocol for Exgene Blood SV mini

Protocol for Exgene Blood SV Midi/Maxi

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Features Blood SV mini Blood SV Midi Blood SV MAXI
Format Column type G (mini) with collection tubeColumn type G (Midi) with collection tubeColumn type G (MAXI) with collection tube
Maximum sample size~200 μl1~2 ml3~10 ml
Preparation time20~30 minutes

40~55 minutes 

40~55 minutes 

Typical yield4~20 μg20~80 μg80~400 μg
Elution volume30~400 μl200~600 μl0.4~2 ml

 

Technical Data

Genomic DNA Extraction from Various Samples

 

 

Compatibility Test with Restriction Enzymes

       

 

PCR Amplification Test

       

 

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Protocols

Protocol for Exgene Blood SV mini

Protocol for Exgene Blood SV Midi/Maxi

 

 

 

 

 

 

 

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

References

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If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200