Cambio - Excellence in Molecular Biology

Extraction & Purification - DNA

Extraction & Purification - DNA: Environmental

Isolate DNA from difficult environmental samples such as soils, compost, clay and sewage.

Exgene™ Plant SV

Exgene™ Plant SV kit provides a simple and easy method for small, medium and large scale purification of total DNA from various plant tissues. With EzSep™ filter and column type G, the procedure can be completed in as little as 40 minutes for the mini-prep version, yielding genomic DNA of high purity which is suitable for use directly in most common downstream appilcations. Up to 100 mg, 400 mg of plant tissue can be processed with Exgene™ Plant SV mini, Midi and MAXI, respectively.

GeneAll

Description

Description

Exgene™ Plant SV kit provides a simple and easy method for small, medium and large scale purification of total DNA from various plant tissues. With EzSep™ filter and column type G, the procedure can be completed in as little as 40 minutes for the mini-prep version, yielding genomic DNA of high purity which is suitable for use directly in most common downstream appilcations. Up to 100 mg, 400 mg of plant tissue can be processed with Exgene™ Plant SV mini, Midi and MAXI, respectively.

 

 

Features and Benefits

¦ Spin or vacuum column format
¦ Stable and consistent DNA extraction from plant cells, tissues and fungi
¦ Instant use : No need of additional materials
¦ Perfect removal of second metabolites such as polyphenols and polysaccharides
¦ Simple procedure by the use of EzSepTM filter column
¦ No use of organic solvents
¦ Ready for use in PCR, Southern blotting, AFLP, RFLP, RAPD and other enzymatic reactions

 

Protocol

          

FeaturesPlant SV miniPlant SV MidiPlant SV MAXI
Format Column type G (mini) with collection tubeColumn type G (Midi) with collection tubeColumn type G (MAXI) with collection tube
Maximum sample size
 ~100 mg wet
(25 mg dry)
~400 mg wet
(100 mg dry)
~1 g wet
(250 mg dry)
Preparation time<40 minutes

<60 minutes 

<60 minutes 

Maximum loading volume750 μl

5 ml

15 ml

Typical yield4~40 μg10~150 μg40~300 μg
Elution volume30~400 μl200~600 μl0.4~2 ml

 

 

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Protocols

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References

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