Description
RibospinTM Plant is specially designed for purification of total RNA from various plant tissues such as leaves, stems, roots and picky plant samples. This kit provides an optimized buffer and spin column, which are very effective in removing polysaccharides and polyphenolic compounds and isolating intact plant RNA. All components of RibospinTM Plant are ready to use, so no further preparation prior to purification required.
The procedure of RibospinTM Plant begins with the disruption of the sample in liquid nitrogen using mortar and pestle. The disrupted sample can be lysed in buffer RPL or REL. In most cases, buffer RPL is the best buffer for lysis. However, in some plant samples, solidification of lysate can occur with buffer RPL due to endosperm of seed or peculiar metabolites and this can be avoided by using buffer REL as alternative to buffer RPL. Most impurities except RNA in the lysate are eliminated by filtration through the EzPureTM filter and then the subsequent lysate is mixed with ethanol to enhance binding.
Total RNA including a small amount of impurities is bound to the membrane of spin column type W while the mixture passes through. Any remaining genomic DNA can be removed by oncolumn DNase I treatment at this step. After a series of washing step using buffer RBW and RNW, plant total RNA is eluted with RNase-free water. The entire procedure for RibospinTM Plant takes only 25 minutes. The purified RNA is suitable for cDNA synthesis, RT-PCR, northern blotting and other analytical procedures.
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Features and Benefits
¦ Glassfiber membrane technology
¦ Sample size: Up to 100 mg plant tissue
¦ Including DNase I and treatment step
¦ Typical yield of RNA : ~ 50 ug / 100 mg tissue
¦ High purity : A260/A280 1.8~2.2, A260/A230 >2.0
¦ Preparation time : ~ 25 minutes
¦ No phenol / chloroform extraction
¦ No ethanol precipitation
¦ Ready for use in RT-PCR, northern blotting, dot blotting, in vitro translation, molecular cloning, real-time
PCR, RNase protection assay and other analytical procedures
Procedures
Specification
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