Type of Sample
• Bacterial suspensions • Isolation of high-copy plasmids: 0.5 – 5 ml • Isolation of low-copy plasmid DNA, P1 constructions, etc.: > 5 - 10 ml
Separation principle
Spin Filter columns
Description
Easy and quick plasmid isolation from bacterial lysis.
Recommended Use
The ExtractNow™ Plasmid Mini Kit is for research use only. Not recommended for clinical applications.
Shelf Life and Storage
Components are maintainable at room temperature for at least 6 months.
Specifications
• Time of Extraction: Approx. 16 minutes • Binding capacity: Column binding capacity: approx. 40 μg pDNA • Average yield: Typical yield from 2 ml starting material (highcopy plasmid): 6 - 20 μg • Average purity: 1.8 - 2.0
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