Food Control™ LFA is a PCR-kit for fast and reliable detection of foodborne pathogens via conventional PCR in combination with our reliable Lateral Flow Assay (LFA) cartrige technology, putting together the easily interpretable LFA stripe and our PCR contamination avoiding LFA cartridge system.
Detecting bacterial contamination is a key aspect in food microbiology. Consequently, microbiological quality control programs are intensified throughout food chain production in order to minimize the risk of intoxicating consumers. Reliability, speed and robustness of a test system to detect the presence or absence, or even the degree of contamination with pathogens, becomes increasingly important. Food Control™ LFA is a diagnostic system for easy non-quantitative detection of presence or absence of foodborne pathogens in agricultural or food industry via conventional PCR and Lateral Flow Cartridge LFA technology.
Type of Assay
Food Control™ PCR-LFA is based on the regular Food Control™ PCR-LFA design, but provides additionally a convenient pre-casted hybridisation cartridge. This system allows the single-use, cross-contamination safe reading of the PCR result for each sample individually.
The LFA cartridge provides the lateral flow stripe and the running buffer required to complete the hybridization and staining process. The PCR tube is inserted into the cartridge. By closing the system a needle taps the tube and the buffer reservoir, instantly starting the flow and the hybridization transferring the PCR amplicons onto the stripe. This one step system reduces preparation and handling steps as well as contamination via e.g. PCR carry over. The result is easily visible through the sight funnel of the cartridge.
Recommended Use / Scope
Can be used in research and industry for detection of pathogenic contamination in food or agricultural products. For research use only! Do not use for clinical diagnostic or testing of patient samples.
The kit is available for the detection of the following microorganisms via conventional PCR:
Features
| Micro-organism |
Target |
| Salmonella enterica |
invasion protein A (invA) gen |
| Yersinia enterocolitica |
heat stable enterotoxin A gen |
| Shigella spp. |
invasion plasmid antigen (ipaH6) gen |
| Campylobacter spp. |
acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
O-acyltransferase (lpxA) gen
|
| Clostridium perfringens |
phospholipase C alpha toxin (plc) gen |
| Shigella dysenteriae, Escherichia coli (EHEC) |
Shiga Toxin 1 (stx1) gen |
| Shigella dysenteriae, Escherichia coli (EHEC) |
Shiga Toxin 2 (stx2) gen |
| Escherichia coli O157 |
wbdR gen |
| Escherichia coli O104 |
wckD gen |
| Listeria spp. |
invasion associated protein p60 (iap) gen |
| Listeria monocytogenes |
listeriolysin O (hly) gen |
| Salmonella spp. |
spacer-region between 16S and 23S RNA genes
|
Result Evaluation
LFA cartridge integrated Lateral Flow Stripe.
Sensitivity
Down to 10 DNA copies/assay.
Content
PCR Mix, rehydration buffer, PCR grade water, positive control, species specific primer, species specific probe, LFA stripe, LFA running buffer, LFA cartridge, LFA cartridge chamber.
Sample Requirements
The Food Control™ kits are designed for the PCR based detection of bacterial DNA. Isolated total DNA from potentially contaminated food serves here as starting material, typically after pre-cultivation of the sample growth medium.
Time to result
150 minutes
Required Consumables
0.2 ml PCR reaction tubes
Optional: DNA can be extracted from enrichment broth using the DNA extraction kit ExtractNow™ Food Control (Cat. No. 609-1050) or user method.
Required Lab Devices
Racks for reaction tubes, benchtop centrifuge for reaction tubes or PCR plates, vortex, variable microliter pipettes (1 µl - 1000 µl), sterile filter tips, any conventional PCR cycler.
Shelf Life and Storage
Components can be stored at 2 to 8 °C for at least 12 months. After rehydration the reagents must be stored at -18 °C.
Compliance
No, certification in progress.
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200