Salt-tolerant DNA & RNA Nuclease HQ is a bioprocessing grade Salt-tolerant DNA & RNA Nuclease. It is the ultimate solution for efficient removal of nucleic acids in manufacturing and bioprocessing workflows. This non-specific, recombinant endonuclease has optimum activity at high salt concentrations, which can improve efficiency and yield in various workflows.
Salt is a key component of various buffers used in protein purification schemes. The presence of salt can minimize protein aggregation, increase target protein solubility and improve target protein yield. High salt enables contaminating DNA to dissociate from associated proteins and become available for degradation. Salt-tolerant DNA & RNA Nuclease high quality (HQ) is highly compatible with the use of high salt conditions.
The main advantages of High Quality
- High purity (≥ 98%)
- No protease detected
- Active at low temperatures
- High activity at high salt conditions
- Supplied with extended product documentation
- Compatible with High Salt nuclease ELISA
Source:
Recombinantly produced in Pichia pastoris
Specific activity: ≥ 175 000 Units/mg
Size: The protein is glycosylated. Protein size without glycosylation is 26 kDa
Unit definition: One Unit is defined as an increase in absorbance at 260 nm of 1 A in 30 minutes at 37°C, using 50 μg/ml calf thymus DNA in a buffer consisting of 25 mM Tris-HCl, pH 8.5 (25°C), 5 mM MgCl2, 500 mM NaCl
Specificity: Nonspecific endonuclease cleaving single- and double stranded DNA and RNA. The enzyme degrades DNA vs. RNA in a 10:1 ratio
End-product: Following complete degradation, the end product consist of a majority of 5 nucleotide oligos
Activity:Tmp: 0–40°C, optimal 35°C
NaCl: 50mM–1M, optimal NaCl is 0.5 M
Mg2 (>1 mM) is required for activity, optimal Mg2 is 20 mM
pH: 7.0–9.5, optimal pH is 9
Tolerance to typical buffer additives:
- Imidazole: 20% activity at 350 mM Imidazole
- Glycerol: 20% activity at 35% glycerol
- Triton X-100: No reduction in activity (tested up to 15%)
- SDS: Not recommended
- Urea: Not recommended
- Reducing agents (e.g. DTT, TCEP): will result in inactivation
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