Highlights
- Compatible with virtually all transfection methods
- Simple application (additive to the cell culture medium)
- Reduces the detection of foreign DNA and RNA through the cell‘s sensors for nucleic acids
- Improves the transfection efficiency and vitality of cells
Eukaryotic cells can detect foreign substances such as
Lipopolysaccharide, bacterial or viral nucleic acids and proteins by the
innate immune system and take defensive measures against the
infiltration of potential pathogenic or initiate apoptotic processes. In
addition, the presence of any cell-damaging substances is communicated
to the neighbouring cells through messengers, accordingly establishing a
defensive attitude, without having direct contact to the pathogen.
The knowledge that virtually all transfection methods are affected of
the defenses of the innate immune system, led to the development of the
K4® Multiplier. Especially hard to transfect cells
often express endosomal or cytosolic sensors that can detect nucleic
acids. In contrast to the K2® Multiplier, the K4® Multiplier
as its further development inhibits both groups of sensors. This is
often the case during transfection of hard to transfect cells by means
of cationic lipids or polymers, electroporation or viral transfection
methods.
In this way, in many cases significant increases transfection
efficiencies combined with improved vitality of the cells can be
achieved, regardless of the applied transfection method. Prerequisite is
that the endosomal or in the cytosol released DNA or RNA play a role
within the transfection process and the cells are expressing the
corresponding sensors.
The K4® multiplier is an additive to the cell
culture medium, easy to use and requiring no optimization. It can
therefore easily be combined with existing optimized transfection
protocols. It is suitable for the transient and stable transfection of
mammalian cell lines and also primary cells with DNA (plasmids,
bacmids), RNA (mRNA, miRNA and siRNA) and modified nucleic acids (such
as antisense oligonucleotides). Application areas are especially the
production of proteins, antibodies and virus (E.g. AAV, adenovirus,
lentivirus), Cotransfectionens of various nucleic acids, genome editing
(E.g. CRISPR/Cas9, CRISPR/Cpf1) and gene silencing (gene knockdown).
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200