Features
- Dissolve and go
- Exactly pre-weighed tablets in bottles
- Mix elution buffer with phosphate buffer to achieve desired imidazole concentration
- Ultrapure, low UV280 absorbance imidazole
- Formulated from analytical grade chemicals
Product description
Immobilized metal ion affinity
chromatography (IMAC) is a widely used separation method for purifying
proteins and peptides that have an affinity for metal ions, such as
histidine-tagged proteins but also some untagged recombinant or native
proteins. Medicago’s IMAC phosphate and elution buffer tablets have been
developed exclusively for use in this method.
IMAC buffer tablets are supplied
in bottles and in blister packs. One tablet of phosphate buffer
dissolved in deionized water yields a 300 mM sodium chloride, 50 mM
sodium phosphate buffer with pH 8.0 at 22°C. One tablet of elution
buffer yields a 300 mM sodium chloride, 300 mM imidazole, 50 mM sodium
phosphate buffer with pH 8.0 at 22°C.
Applications
- Purification of histidine-tagged and non-histidine-tagged recombinant and native proteins using IMAC chromatography
- The phosphate buffer may be used for other types of affinity chromatography methods
Directions for use
Prepare the phosphate and the
elution buffers separately. Deposit one tablet in a laboratory flask or
beaker placed on a magnetic stirrer. Add deionized water up to the given
volume for each tablet and stir until full dissolution. Mix the
phosphate buffer and elution buffer to achieve desired imidazole
concentration, follow the dilution series included in the product sheet.
Shipping and storage
IMAC phosphate and elution buffer
tablets are shipped at room temperature. Store the tablets in a dry
place at room temperature. Shelf life for IMAC phosphate buffer tablets
is five years and shelf life for IMAC elution buffer tablets is three
years.
Specifications
Chemicals: Analytical grade
Format: Exactly pre-weighed tablets
Composition:
Phosphate Buffer
300 mM Sodium Chloride
50 mM Sodium Phosphate
Elution Buffer
300 mM Sodium Chloride
300 mM Imidazole
50 mM Sodium Phosphate
Volume:
Phosphate Buffer: 400 ml
Elution Buffer: 100 ml
pH 8.0 ± 0.05 at 22°C
Shelf life:
Phosphate Buffer: 5 years after production date
Elution Buffer: 3 years after production date
MSDS:
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200