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Oligo Synthesis

Oligo Synthesis : CEPs

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beta-L-DNA Phosphoramidites

beta-L-DNA Phosphoramidites

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-2101-02beta-L-Pac-dA-CE Phosphoramidite0.25 g £136.00 Quantity Add to Order
10-2101-05beta-L-Pac-dA-CE Phosphoramidite0.5 g £273.00 Quantity Add to Order
10-2101-10beta-L-Pac-dA-CE Phosphoramidite1.0 g £545.00 Quantity Add to Order
10-2115-02beta-L-Ac-dC-CE Phosphoramidite0.25 g £68.00 Quantity Add to Order
10-2115-05beta-L-Ac-dC-CE Phosphoramidite0.5 g £136.00 Quantity Add to Order
10-2115-10beta-L-Ac-dC-CE Phosphoramidite1.0 g £273.00 Quantity Add to Order
10-2121-02beta-L-iPr-Pac-dG-CE Phosphoramidite0.25 g £136.00 Quantity Add to Order
10-2121-05beta-L-iPr-Pac-dG-CE Phosphoramidite0.5 g £273.00 Quantity Add to Order
10-2121-10beta-L-iPr-Pac-dG-CE Phosphoramidite1.0 g £545.00 Quantity Add to Order
10-2130-02beta-L-dT-CE Phosphoramidite0.25 g £68.00 Quantity Add to Order
10-2130-05beta-L-dT-CE Phosphoramidite0.5 g £136.00 Quantity Add to Order
10-2130-05beta-L-dT-CE Phosphoramidite0.5 g £136.00 Quantity Add to Order
10-2130-10beta-L-dT-CE Phosphoramidite1.0 g £273.00 Quantity Add to Order

Description

Beta L-DNA is the mirror image version of naturally occurring D-DNA. L-DNA and D-DNA share identical structures that differ only in terms of stereochemistry and generally have identical physical and chemical properties. The difference in their stereochemistry results in differences in their interactions with chiral molecules, D-DNA will only bind to its D-DNA complement to form right-handed helices, and likewise, L-DNA will only bind to its L-DNA complement to form left-handed helices. For this reason, enzymes that interact with D-DNA, including nucleases, typically won’t interact with L-DNA. The unique properties of L-DNAs have made them attractive for many biological applications such as Aptamers, Molecular Beacons, Molecular Tagging, and Drug Nanocarriers. Note that the procedure for synthesizing L-DNA oligonucleotides is very similar to that of D-DNA oligonucleotides. Please see GR31.2 for more details.

 

beta-L-Ac-dC-CE Phosphoramidite

5'-Dimethoxytrityl-N-acetyl-beta-L-2'-deoxyCytidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

 

beta-L-Pac-dA-CE Phosphoramidite

5'-Dimethoxytrityl-N-phenoxyacetyl-beta-L-2'-deoxyAdenosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

 

beta-L-iPr-Pac-dG-CE Phosphoramidite

5'-Dimethoxytrityl-N-p-isopropyl-phenoxyacetyl-beta-L-2'-deoxyGuanosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

 

beta-L-dT-CE Phosphoramidite

5'-Dimethoxytrityl-beta-L-2'-deoxyThymidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Protocols

Downloead SDS here (from supplier's website)

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

References

  1. K.P. Williams, et al., Proc Natl Acad Sci U S A, 1997, 94, 11285-11290.
  2. A. Vater, and S. Klussmann, Drug Discov Today, 2015, 20, 147-155.
  3. Y. Kim, C.J. Yang, and W. Tan, Nucleic Acids Res., 2007, 35, 7279-7287.
  4. G. Hayashi, M. Hagihara, and K. Nakatani, Nucleic Acids Symp Ser (Oxf), 2005, 261-262.
  5. K.-R. Kim, T. Lee, B.-S. Kim, and D.-R. Ahn, Chemical Science, 2014, 5, 1533-1537.

 

See also Glen Report 31.2

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Applications & Benefits

The unique properties of L-DNAs have made them attractive for many biological applications such as Aptamers, Molecular Beacons, Molecular Tagging, and Drug Nanocarriers. Note that the procedure for synthesizing L-DNA oligonucleotides is very similar to that of D-DNA oligonucleotides. Please see GR31.2 for more details.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200