A GREAT INTRO TO ELECTROPHORESIS AND MOLECULAR SEPARATION.
In this miniPCR Learning Lab, students identify the molecules found
in a mixture of dyes using two molecular separation techniques. Students
will learn the key principles of gel electrophoresis and paper
chromatography, and compare the two techniques. This lab introduces
students to key concepts about molecules and molecular properties that
are relevant to future discussions of important biological
macromolecules such as DNA and proteins.
Students will explore how physicochemical properties (mainly size and
charge) of molecules influence their migration through an agarose gel
or in a paper chromatography separation. They will make predictions
about the molecular composition of unknown dye mixtures based
on empirical results. These experiments are highly visual and lend
themselves to engage students of diverse learning styles.
This Learning Lab is suitable for students from middle school to AP
Biology, and it is aligned with National Standards. It utilizes the
following molecular genetic techniques and analyses:
- Micropipetting
- Gel electrophoresis
- Paper chromatography
- Use of charge/size ratios in the analysis of macromolecules
- Use observation to inform predictions about unknown molecular samples
The blueGel™ Electrophoresis Rainbow Lab kit contains reagents for 8 lab groups of 4 students each (32 students):
- 5 dyes of different molecular characteristics
- Chromatography strips and accessories for chromatography
- Contrast-enhancing paper for easier visualization of results
- Reagents are compatible with blueGel™ or other standard horizontal electrophoresis systems
Please note: microtubes, agarose, and electrophoresis buffer sold separately.
Free miniPCR Learning LabTM downloads:
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200