Cambio - Excellence in Molecular Biology

Lab Equipment

Lab Equipment: Benchtop Equipment

This section includes equipment from Amplyus: the miniPCR machine, minicentrifuges and the Bluebox, a cost-effective blue light box for visualisation of DNA, RNA or protein using fluorescent dyes.

Kilobaser Express Benchtop DNA & RNA Synthesizer

Synthesize your RNA and DNA oligos in just 2 hours!

Kilobaser

Description

 

WORLD’S FIRST
CARTRIDGE BASED
SYNTHESIZER

 

 

 

 

 

 

KILOBASER EMPOWERS ANY LAB PROFESSIONAL TO CREATE DNA & RNA.

 

Printing your DNA and RNA oligos becomes as easy as brewing a cup of coffee – less waiting, more time for
science. Your Kilobaser benchtop synthesizer automatically synthesizes ready-to-use DNA and RNA oligos and
probes in less than 2 hours. Suitable for all research and development applications.

 

BENCHTOP DNA SYNTHESIS

 

  • Kilobaser is a benchtop DNA  and RNA synthesizer. It‘s designed to be operated by anyone, there‘s no
    special training needed
  • Our reagent cartridge holds reagents for
    150 DNA bases. It can be used for up to 2
    weeks after activation.
  • Each synthesis starts with our single-use
    Kilobaser microfluidic chip and ends with
    our DNA in a standard PCR-style 0.2 mL tube.
  • The very affordable price makes the Kilobaser
    instrument highly accessible for any lab.
  • Connect to the benchtop DNA synthesizer using
    any mobile or desktop device or use the screen
    provided.
  • To protect your data we ensured Kilobaser
    can be operated without internet connection.

 

 

 

 

 

MAKE FLUORESCENT DNA PROBES WITH KILOBASER

 

  • TaqMan dual-label probes anneal within a DNA region amplified by a specific set of primers. As the DNA
    polymerase extends from the primer and synthesizes the resulting strand, its 5’-3’ exonuclease activity
    cleaves the hybridized probe.
  • The quencher suppresses fluorescencevia the FRET mechanism. Degradation of the probe liberatesthe reporter dye, thus inactivating thequenching effect and allowing the fluorophore to emit light when excited by UV light.
  • Hence, fluorescence detected in the qPCR is directly proportional to the fluorophore released and the amount

 HARD FACTS

 Synthesis duration Yield Additional requirements
 2,5 minutes per base
30 minutes post processing
Length
up to 50 bases
Typical probe: 18-30 bases		 200 pmol
Stepwise yield 99,5% (per base		 Nitrogen and argon gas supply
needed (bottle or house line)

MODIFIED RNA IN NO TIME

 

As with the COVID-19 mRNA vaccine, RNA-based drug research is experiencing an upswing. Not only vaccines, but also cancer and other metabolic diseases are on the target of RNA research. Ribozymes, aptamers, small interfering RNA - RNA has many names that indicate its various functions in the cell. Many functions result in many possibilities to intervene in a disturbed cell process and to steer it back on the right track.

 

 

 


 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
View a quick 45 second video to see how easy it is to produce your own oligos in under 60 minutes

 


 

 
 
 
 
 
TECHNICAL SPECS

Producing your own DNA primers becomes as convenient as brewing a nice cup of coffee

Click here to download product brochure


 

OLIGO QUALITY ANALYSIS

Click on the image to enlarge

  

 

View more videos of the kilobaser below

BENEFITS


ACCELERATION

Accelerate your research and develop new flows. No need to wait days or even weeks. You can have oligos in just a few hours.

 

CONVENIENCE

Kilobaser is as easy to use as it can get. Load the device with its single reagent cartridge, insert a new fluid chip and you're ready to enter your desired DNA sequence.

 

SECURITY

Your DNA data stays strictly in-house. 

 

HARD FACTS

Synthesis duration : 2.4 minutes per base 15 minutes post processing. High-purity oligos: 45 seconds per base
Length: Up to 80 bases
Yield: 300 pmol
Additional requirement: Argon or nitrogen gas supply needed (bottle or houseline)

 

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Protocols

Click here for FAQS

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Notes

 

HOW TO MAKE FLUORESCENT PROBES WITH KILOBASER

1  Have a Kilobaser in your lab?
 
If you don't have a Kilobaser yet, schedule an online demo right away and get a tailored offer.

2  Use FAM cartridge for 3' labeling
 
By combining the FAM cartridge with our standard chips you can make single labeled probes for e.g. FISH assays or LightCycler FRET donor probes.

3  Combine with BHQ-1 quencher chip for dual labeled probes

 

 

HOW TO MAKE RNA ON THE KILOBASER

Use 2'-MOE cartridges to make RNAs for RNA therapeutics applications

 

Just ask tech support any questions you might have at tech@cambio.co.uk

 

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Applications & Benefits

 

Read the white paper on making fluorescent probes for qPCR, FISH and more here:

KILOBASER WHITEPAPER PDF

FAQs here

 

 

 

 

 

 

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200