Description
AquaPhluor® 639 is a rhodamine dye
with an excitation and emission in the far red of the visible spectrum
and is a logical alternative to Cyanine 5. Like Cyanine 5, AP639 is
relatively bright and exhibits sequence- and pH-independent
fluorescence. Unlike Cyanine 5, AP639 also shows excellent chemical and
photochemical stability as well as temperature-independent fluorescence.
AP639 is available as the phosphoramidite and CPG for 5’ and 3’
labeling, respectively.
Details
Usage
- Coupling: 3 minute coupling time recommended
- Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
| Diluent | Anhydrous Acetonitrile |
| Storage |
Freezer storage, -10 to -30°C, dry |
| Stability | 1-2 Days |
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
| Catalog # |
Pack Size |
Grams/Pack |
0.1M Dil. (mL) |
Approximate Number of Additions |
|
LV40 |
LV200 |
40nm |
0.2μm |
1μm |
10μm |
| 10-5926-95 |
50 µmol |
0.06 |
0.5 |
3.33 |
2 |
1.25 |
0.91 |
0.67 |
0.17 |
| 10-5926-90 |
100 µmol |
0.13 |
1 |
20 |
12 |
7.5 |
5.45 |
4 |
1 |
| 10-5926-02 |
0.25 g |
0.25 |
1.96 |
52 |
31.2 |
19.5 |
14.18 |
10.4 |
2.6 |
Expedite
| Catalog # |
Pack Size |
Grams/Pack |
Dilution (mL) |
Approximate Number of Additions |
|
Molarity |
50nm |
0.2μm |
1μm |
15μm |
| 10-5926-95E |
50 µmol |
0.06 |
0.75 |
0.07 |
8.6 |
5.38 |
3.91 |
0.54 |
| 10-5926-90E |
100 µmol |
0.13 |
1.5 |
0.07 |
23.6 |
14.75 |
10.73 |
1.48 |
| 10-5926-02E | 0.25 g | 0.25 | 2.93 | 0.07 | 52.2 | 32.63 | 23.73 | 3.26 |
Intellectual Property
This product is for research purposes
only, and may not be used for commercial, clinical, diagnostic or any
other use. The Product is subject to proprietary rights of ELITechGroup
and is made and sold under license from ELITechGroup. There is no
implied license for commercial use with respect to this Product and a
license must be obtained directly from ELITechGroup. with respect to any
proposed commercial use of this Product. "Commercial use" includes but
is not limited to the sale, lease, license or other transfer of the
Product or any material derived or produced from it, the sale, lease,
license or other grant of rights to use the Product or any material
derived or produced from it, or the use of the Product to perform
services for a fee for third parties (including contract research).
AquaPhluor®, Yakima Yellow®, Redmond Red® and Eclipse® are registered
trademarks of ELITechGroup.
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200