5'-Biotin II Phosphoramidite
[1-N-(4,4'-Dimethoxytrityl)-biotinyl-6-aminoethoxyethyl]-2-cyanoethyl-(N,N-diisopropyl)-phosphoramidite
Product Specifications
F.W.:
393.39
Description
Biotin is a popular oligonucleotide
modification and is used in a wide range of applications, including
enrichment, detection, and immobilization assays. 5’-Biotin II consists
of an ethylene glycol linker, an alternative structure to our
traditional version. 5’-Biotin II phosphoramidite can be added ONLY ONCE
to the 5'-terminus of an oligonucleotide. However, the DMT group on the
biotin can be used in RP cartridge and HPLC purification techniques.
Details
Usage
- Coupling: 2 minute coupling time recommended
- Deprotection:
5'-Biotin is slow to detritylate. If the final DMT-group is to be
removed on the synthesizer, we recommend a second deblocking step. If
the final DMT-group is left on to aid in cartridge purification, we
recommend that the oligo is left in contact with the TFA solution for 10
minutes.
| Specifications |
|---|
| Diluent |
Anhydrous Acetonitrile |
| Storage |
Storage: Freezer storage, -10 to -30°C, dry |
| Stability | 1-2 Days |
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
| Catalog # |
Pack Size |
Grams/Pack |
0.1M Dil. (mL) |
Approximate Number of Additions |
|
LV40 |
LV200 |
40nm |
0.2μm |
1μm |
10μm |
| 10-1954-02 |
0.25 g |
0.25 |
3 |
86.67 |
52 |
32.5 |
23.64 |
17.33 |
4.33 |
| 10-1954-90 |
100 µmol |
0.08 |
1 |
20 |
12 |
7.5 |
5.45 |
4 |
1 |
| 10-1954-95 |
50 µmol |
0.04 |
0.5 |
3.3 |
2 |
1.25 |
0.91 |
0.67 |
0 |
Expedite
| Catalog # |
Pack Size |
Grams/Pack |
Dilution (mL) |
Approximate Number of Additions |
|
Molarity |
50nm |
0.2μm |
1μm |
15μm |
| 10-1954-02E |
0.25 g |
0.25 |
4.47 |
0.07 |
83 |
51.88 |
37.73 |
5.19 |
| 10-1954-95E |
50 µmol |
0.04 |
0.07 |
0.07 |
8.6 |
5.38 |
3.91 |
0.54 |
| 10-1954-90E | 100 µmol | 0.08 | 1.5 | 0.07 | 23.6 | 14.75 | 10.73 | 1.48 |
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