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Oligo Synthesis

Oligo Synthesis : CEPs

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DEACM Caged-dG-CE Phosphoramidite

5'-Dimethoxytrityl-N2-(4-isopropylphenoxyacetyl)-O6-[[7-(diethylamino)coumarin-4-yl]-methyl]-2'-deoxyGuanosine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1533-02DEACM Caged-dG-CE Phosphoramidite0.25g £750.00 Quantity Add to Order
10-1533-90DEACM Caged-dG-CE Phosphoramidite100µmoles £360.00 Quantity Add to Order
10-1533-95DEACM Caged-dG-CE Phosphoramidite50µmoles £180.00 Quantity Add to Order

Description

Photocaged oligonucleotides allow for the spatial temporal control of biological processes. In their caged form, the oligonucleotides are unable to participate in base pairing. It is only after exposure to non-invasive light that the native oligonucleotide is released. DEACM-dG is a convenient way to introduce a photocage at specific positions, and the DEACM is an effective photocleavable group.

Product Specifications

Formula: C65H75N8O11P
M.W.: 1175.31
F.W.: 558.49 (DEACM-dG), 329.21 (dG)
CAS Number: 1325717-65-4

Product Specifications

Formula:
C65H75N8O11P
M.W.:
1175.31
F.W.:
558.49 (DEACM-dG), 329.21 (dG)
CAS Number:
1325717-65-4

Usage

  • Coupling: 3 minute coupling time recommended.
  • Deprotection: If UltraMILD reagents are used, deprotect in 0.05M Potassium Carbonate in Methanol overnight at Room Temperature. If standard bases are used, deprotect in Ammonium Hydroxide at Room Temperature overnight (dmf-dG required) or 30% ammonia/40% methylamine 1:1 (AMA) at Room Temperature for 2 hours (Ac-dC required). Heat should be avoided, both during deprotection and processing.
Specifications
Diluent Anhydrous Acetonitrile
Storage Storage: Freezer storage, -10 to -30°C, dry
Stability1-2 Days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions

LV40 LV200 40nm 0.2μm 1μm 10μm
10-1533-95 50 µmol 0.06 0.5 3.33 2 1.25 0.91 0.67 0.17
10-1533-90 100 µmol 0.12 1 20 12 7.5 5.45 4 1
10-1533-02 0.25 g 0.25 2.13 57.67 34.6 21.63 15.73 11.53 2.88

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions

Molarity 50nm 0.2μm 1μm 15μm
10-1533-95E 50 µmol 0.06 0.75 0.07 8.6 5.38 3.91 0.54
10-1533-90E 100 µmol 0.12 1.5 0.07 23.6 14.75 10.73 1.48
10-1533-02E0.25 g0.253.170.075735.6325.913.56

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

References

Safety Data Sheet

Glen Report 34-11: Beyond the UV Region — DEACM-dG as a Versatile Tool for Light-Activatable (“Caged”)

OligonucleotidesGlen Report 34-12: New Product — DEACM Caged-dG

 

References

The Glen Report, 2011, 23.2, 7.

H. Lusic, D.D. Young, M.O. Lively, and A. Deiters, Org Lett, 2007, 9, 1903-6.

D.D. Young, H. Lusic, M.O. Lively, J.A. Yoder, and A. Deiters, Chembiochem, 2008, 9, 2937-40.

J. Hemphill, and A. Deiters, J Am Chem Soc, 2013, 135, 10512-8.

Y. Liu, et al., Science, 2020, 368, 1265-1269.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

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If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200