Product information
T4 DNA Ligase catalyses the formation of a phosphodiester bond between
the terminal 5′ phosphate and 3′ hydroxyl groups of duplex DNA or RNA.
The enzyme efficiently joins blunt and cohesive ends and repairs single
stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
| T4 DNA Ligase Version |
Sizes |
Ligase Concentration |
Provided Buffer |
| Low Concentration Ligase |
1,500 U
7,500 U
|
2 U/µL |
10X T4 DNA Ligase Buffer |
| High Concentration Rapid Kit |
1,500 U
7,500 U |
10 U/µL |
2X Rapid Ligation Buffer,
10X T4 DNA Ligase Buffer
|
10X T4 DNA Ligase Buffer is composed of 500 mM Tris-HCI, 100 mM MgCl 2, 50
mM dithiothreitol, 10 mM ATP, pH 7.6 @ 25 °C.
2X Rapid Ligation Buffer is composed of 132 mM Tris-HCI, 20 mM MgCl 2, 2 mM dithiothreitol, 2 mM ATP, 15% PEG, pH 7.6 @ 25 °C.
< Figure 1: Purity
The purity of the T4 DNA ligase is equal or greater
than 95% as judged by SDS-polyacrylamide gel electrophoresis with
Coomassie blue staining. Molecular weight markers are in Lane 1. T4 DNA
ligase was loaded at 5 units (Lane 2) and 10 units (Lane 3).
>Figure 2: Exonuclease and Endonuclease Activity Assay
The T4 DNA ligase is free of detectable of exo- and endonuclease
activities, as judged by agarose gel electrophoresis following
incubation of 10 units of enzyme for 16 hours at 37 °C with 1 µg of
HindIII-digested λ DNA (Lanes 3 and 4) and supercoiled pUC19 DNA (Lanes 5
and 6). Molecular weight markers are in Lanes 1 and 2.
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