Cambio - Excellence in Molecular Biology

Cell Culture Contamination Control

Cell Culture Contamination Control: Detection

Mycoplasma Detection

As an efficient contamination control measure we recommend frequent routine testing of cell culture materials with Venor®Gem Mycoplasma Detection Kits. These PCR- and qPCR-based mycoplasma assays allow fast (within a few hours), simple, effective and sensitive detection of mycoplasma-contaminated cell cultures in any standard molecular biology laboratory.

Regular mycoplasma testing and control procedures can significantly help in avoiding the costly consequences of contamination ensuring safe biopharmaceuticals and reliable scientific results.  Many scientific journals currently do not accept submissions of cell-based studies without evidence that mycoplasma testing has been performed.

To meet different customer needs Minerva Biolabs has developed a range of Venor®GeM mycoplasma detection assays using conventional PCR or qPCR methods which enable rapid and reliable identification of mycoplasma in cell cultures, culture supernatants and tissue culture media.

Conventional PCR

  • Venor®GeM Classic allows fast, reliable and time-saving routine monitoring of mycoplasma contamination in cell culture supernatants, media and biopharmaceuticals in research and industry by conventional PCR.

  • Venor®GeM OneStep is a kit for direct detection. It requires minimal pipetting, the kit includes all reagents required for PCR in a ready-to-use lyophilized reaction mix. Only samples or controls need to be added prior to PCR.

  • Venor®GeM Advance is designed for direct detection. Designed to reduce the total assay time and the pipetting steps, the kit contains PCR strips of tubes, each pre-dispensed with all reagents necessary for each PCR reaction, including the polymerase. For additional convenience, the gel loading buffer and dye are already included in the reaction buffer. After PCR, the products can be loaded directly on an agarose gel.

Real-time PCR (qPCR)

Our qPCR kits are TaqMan®-based qPCR Assays with labelled probes:  FAM® channel (mycoplasma) and HEX® channel (internal control to check for PCR inhibition from contaminants in sample).  So not only does the qPCR approach remove a lot of hassle, but the internal controls also give you reassurance that your negatives are not false.

  • Venor®GeM qEP utilises quantitative, real-time PCR (qPCR) for fast (approx. 3 h) and reliable screening of cell culture supernatants for mycoplasma contamination. The kit can be used in combination with any type of real-time PCR cycler able to detect the fluorescence dyes FAM® and HEX®. For clinical diagnostics in which human samples are tested we recommend using this kit as it conforms to EP 2.6.7, USP <71> and JP G3. It is suitable for both research and industry where certified compliance is needed or desirable. 

  • Venor®GeM qOneStep is a kit based on a real-time PCR (qPCR) assay for rapid, robust and sensitive detection of mycoplasma contamination. The detection procedure can be performed within 3 hours. Primers, nucleotides, probes, polymerase and an internal amplification control are provided as a ready-to-use, lyophilized reaction mix. This kit is a very straightforward and is highly suitable for academic labs and industrial applications where certified compliance of kits is not required.


Venor®GeM SP Kit - Beads

The Venor®GeM SP Kit - Beads is a DNA/RNA extraction kit designed to separate nucleic acids from mycoplasma. The Venor®GeM SP Kit - Beads is based on the extraction of DNA/RNA using magnetic beads. In mycoplasma contamination control and detection, different samples such as cell culture suspensions or cryostocks are analysed. The cell culture supernatants to be tested contain different factors that can lead to inhibition of PCR...

Minerva Biolabs

Catalogue No.DescriptionPack SizePriceQty
56-3010Venor®GeM SP Kit - Beads10 extractions £110.00 Quantity Add to Order
56-3100Venor®GeM SP Kit - Beads100 extractions £425.00 Quantity Add to Order

Description

Venor®GeM SP Kit - Beads

 

The Venor®GeM SP Kit - Beads is a DNA/RNA extraction kit designed to separate nucleic acids from mycoplasma. The Venor®GeM SP Kit - Beads is based on the extraction of DNA/RNA using magnetic beads. In mycoplasma contamination control and detection, different samples such as cell culture suspensions or cryostocks are analysed. The cell culture supernatants to be tested contain different factors that can lead to inhibition of PCR. Factors such as a large number of proteins in the cell culture suspension or a to high confluency of more than 80-90% have a major inhibiting influence on nucleic acid amplification techniques (NAT). This is where our Venor®GeM SP Kit - Beads comes in. The Venor®GeM SP Kit - Beads, with its fast performance and high extraction efficiency, is adapted to our products of the Venor®GeM series, such as the Venor®GeM qEP (Art.-No.: 11-9025) or also the Venor®GeM qOneStep (Art.-No.: 11-91025), for the detection of mycoplasma.

 

 

Excellent reproducibility and quality due to automatic extraction

The Venor®GeM SP Kit - Beads is also compatible with extraction robots such as the KingFisher™ Flex. Its automated nucleic acid extraction significantly improves the reproducibility of individual extractions. Even when increasing the throughput by increasing the number of samples up to 96 samples simultaneously, the DNA/RNA extraction of the Venor®GeM SP Kit - Beads maintains a constant quality. The ready-to-use setup program for the KingFisher™ Flex can be downloaded - see link in manual.

Recommended Use

For DNA/RNA extraction from:

  • Cell culture supernatants
  • Cell suspensions
  • Cryostocks
  • Biopharmaceuticals

Kit Components

  • Binding, Elution, Lysis, Rehydration and Wash 1-3 Buffers
  • Magnetic Bead Suspension
    Proteinase K

Required Consumables

  • 1-propanol ≥ 99,5% (required for the reconstitution of the Binding Buffer and the Wash Buffer 1 and 2)
  • RNase-free 1.5 ml or 2 ml reaction tubes (preferably low-bind)
  • Serological pipettes and pipettes with corresponding filter tips (100 and 1000 μl)

Required Lab Devices

For manual extraction:

  • Magnetic rack and thermoshaker for 1.5 ml (or 2 ml) reaction tubes
  • Optional: Microcentrifuge

For automatic extraction:

  • Automatic extractor e.g. KingFisher™ Flex
  • Plastic material (deep-well plates and elution plate)

Package Sizes

  • Cat. No. 56-3010 10 extractions
  • Cat. No. 56-3100 100 extractions

Storage

Ship and store at ambient temperature.
Attention: Remove the Proteinase K and store it at 4 °C upon receipt of the Venor®GeM SP Kit - Beads.

Manual.pdf
SDS.pdf
Short instructions Short instructions.pdf Short instructions.pdf

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200