Cambio - Excellence in Molecular Biology

Lab Reagents

Lab Reagents: Absolute Lectins

Absolute Lectins, perfect for Glycoscience Medicago is a primary manufacturer of a wide variety of exceptionally pure lectins purified by affinity chromatography. Lectins are non-enzymatic proteins of non-immunoglobulin origin that bind specifically and reversibly to carbohydrate moieties without altering the covalent structure of the glycosyl ligands. The term ‘lectin’ is derived from the latin word legere, meaning ‘to select’. The specificity of a lectin is usually defined by the monosaccharides or oligosaccharides that are best at inhibiting the agglutination or precipitation caused by the lectin. Lectins are usually of plant origin but do occur in many types of organism; some are glycoproteins and may be soluble or membrane-bound.

The biological function of lectins is far from fully explored. Their specificity enables binding to glycoproteins and polysaccharides as well as agglutination of erythrocytes and stimulation of blood lymphocytes. Because of their ability to distinguish glycosyl ligands on human red blood cells, lectins can be used for blood typing.Immobilized lectins can be used in affinity chromatography to purify and isolate glycoproteins, glycolipids, polysaccharides, viruses and cells.

Carbohydrate-containing substances bound to the lectin may be eluted with a competitive binding substance. Medicago offers lectins as a freeze-dried powder or as a solution manufactured under aseptic conditions. If you don’t find the lectin you are looking for, we can certainly produce it for you. Furthermore, we offer different custom lectin conjugates on request.

•Ultrapure quality

•High activity

•Wide range

•Lyophilized powder

•Custom lectins and conjugates

Wisteria floribunda lectin (WFL)

"Wisteria floribunda lectin is isolated from japanese wisteria seeds and purified by affinity chromatography. The lectin has a molecular weight of 116 kDa and is composed of four polypeptide chains of 29 kDa.

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
MC-05-0041-100Wisteria floribunda lectin (WFL)100 mg £895.00 Quantity Add to Order
MC-05-0041-5Wisteria floribunda lectin (WFL)5 mg £131.00 Quantity Add to Order

Description

Wisteria floribunda lectin (WFL)

*** For pack size 1g or more, please contact us to inquire about pricing


Features

  • Ultrapure quality
  • Sugar specificity: N-acetyl galactoseamine, galactose, complex oligosaccharides containing N-acetylgalactoseamine
  • Haemagglutinating activity
  • Lyophilized powder

 


Product description

"Wisteria floribunda lectin is isolated from japanese wisteria seeds and purified by affinity chromatography. The lectin has a molecular weight of 116 kDa and is composed of four polypeptide chains of 29 kDa.

 

The lectin labels selectively N-acetylgalactosamines beta 1 (GalNAc beta 1-3 Gal) residues of glycoproteins within the extracellular matrix of the neurons, and has been identified as a specific marker for functionally different cortical areas of the rodent brain. Wisteria floribunda lectin can also be used as a probe for serum biomarkers of several diseases such as liver fibrosis, liver cirrosis, IgA nephropathy, entometriosis and a long list of cancer types, with very low background noise.

 

Medicago’s Wisteria floribunda lectin is supplied as a white to yellowish-white lyophilized powder. The purity is determined by SDS- PAGE, which generates one single band at 29 kDa corresponding to the four polypeptide chains. The lectin is available in vials containing 5 mg or 100 mg powder, but for bulk purchases other packaging can be prepared. The product is to be used for laboratory work only."

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200