Target: Botulinum toxin (BoNT-B)
Target Species: bacterial
Target Type: protein
Affinity Constant (Kd): 3.2 nM
Default 3' Linker (Spacer) is 10T. Please submit a custom quote request for other options.
Identifiers: BoNT-B aptamer #983
- Number of DNA nucleotides: 32 bases
- Molecular weight: 10,412.1 g/mol
- Target for selection: Botulinum Neurotoxin Type B, Metabiologics Inc.
Aptamer was selected from a randomized 32-mer library against BoNT-B protein. Proprietary methods were then used to select the aptamer. Aptamer folding instruction before use: Once the aptamer is in its working concentration, it needs to be heated to 85-90 ºC for 2 minutes, and then cooled to room temperature before use.
Validation data with BoNT-B by MST (Microscale Thermophoresis) method:
Buffer used for validation: 1X PBS, pH = 7.4, 1 mM MgCl2
Average Kd = 3.2 ± 0.7nM
Please choose your modification and add as an extra catalogue number:
ATWUNMOD - Unmodified
ATWAMINE - Amine modification
ATWBIOTIN - Biotin modification
ATWTHIOL - Thiol modification (extra charge)
ATWFLUOR - Fluor (all) modification (extra charge)
Custom aptamers can be developed; contact us for details
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