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Aptamers

Aptamers: Catalogue Aptamers

Please remember to pick your Modification from "Related items"

If the listed modifications do not meet your needs many other modifications are available on request

All Aptamers from Base Pair Bio are available with the following modifications

Please choose your modification and add as an extra catalogue number:

 

ATWUNMOD - Unmodified

ATWAMINE - Amine modification

ATWBIOTIN - Biotin modification

ATWTHIOL - Thiol modification (extra charge)

ATWFLUOR - Fluor (all) modification (extra charge)

 

BoNT-B

Botulinum Neurotoxin Type B Aptamer ATW0075

Base Pair Bio

Catalogue No.DescriptionPack SizePriceQty
ATW0075-100BoNT-B 10 AEU (100ug) POA Quantity Add to Order
ATW0075-25BoNT-B 2.5 AEU (25ug) POA Quantity Add to Order

BoNT-B

Botulinum Neurotoxin Type B Aptamer ATW0075

Base Pair Bio

Target:   Botulinum toxin (BoNT-B)

Target Species:   bacterial

Target Type:   protein

Affinity Constant (Kd):   3.2 nM

Default 3' Linker (Spacer) is 10T. Please submit a custom quote request for other options.

Datasheet

Identifiers: BoNT-B aptamer #983

  • Number of DNA nucleotides: 32 bases
  • Molecular weight: 10,412.1 g/mol
  • Target for selection: Botulinum Neurotoxin Type B, Metabiologics Inc.

Aptamer was selected from a randomized 32-mer library against BoNT-B protein. Proprietary methods were then used to select the aptamer. Aptamer folding instruction before use: Once the aptamer is in its working concentration, it needs to be heated to 85-90 ºC for 2 minutes, and then cooled to room temperature before use.

Validation data with BoNT-B by MST (Microscale Thermophoresis) method:

Buffer used for validation: 1X PBS, pH = 7.4, 1 mM MgCl2

Average Kd = 3.2 ± 0.7nM

Please choose your modification and add as an extra catalogue number:
ATWUNMOD - Unmodified
ATWAMINE - Amine modification
ATWBIOTIN - Biotin modification
ATWTHIOL - Thiol modification (extra charge)
ATWFLUOR - Fluor (all) modification (extra charge)

Custom aptamers can be developed; contact us for details

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

BoNT-B

Botulinum Neurotoxin Type B Aptamer ATW0075

Base Pair Bio

Please note that aptamers have much lower molecular weights than antibodies. Thus, there are typically five to fifteen times as many moles or molecules of aptamers present in a solution containing an equal mass. For example, 100 micrograms of an aptamer is equivalent to 0.5 to 1.5 mg of antibody, depending on the particular aptamer.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200