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Enzymes for Molecular Biology

Enzymes for Molecular Biology: Enzymes for Molecular Biology

Exo-Minus Klenow DNA Polymerase

Klenow DNA Polymerase Exo- is a DNA-dependent DNA polymerase that lacks both the 5'-3' and 3'-5' exonuclease activities of E. coli DNA Polymerase I from which it is derived.

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
E0141-10D3Klenow DNA Polymerase Exonuclease Minus (D355A, E357A), 2 mL2ml POA Quantity Add to Order
KL11101KExo-Minus Klenow DNA Polymerase (D355A, E357A)1kU 10U/ul £261.00 Quantity Add to Order
KL11120KKlenow DNA Polymerase Exonuclease Minus Kit (D355A, E357A),10K U at 20KU/ml POA Quantity Add to Order

Description

Klenow DNA Polymerase Exo- is a DNA-dependent DNA polymerase that lacks both the 5'- 3' and 3'- 5' exonuclease activities of E. coli DNA Polymerase I from which it is derived. 

Concentration and Specific Activity

20 Units /µl at greater than 1 X 104 Units/mg 

Unit Definition

One Unit of Klenow DNA Polymerase Exo- converts 10 nmoles of dNTPs into acid-insoluble material in 30 minutes at 37°C. 

Quality Control

Klenow DNA Polymerase Exo- is free of detectable endo- and exonuclease and RNase activities.



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Protocols

Protocols for: Klenow DNA Polymerase Fragment Exo-

 

Due to the constant updating of the protocols by the manufacturer we have provided a direct link to Lucigen’s product page, where the latest protocol is available.

Please note this will open a new page or window on your computer.

/library/images/html_images/2022 protocols/KL11101K_Exo-Minus-Klenow.pdf

(catalogue number KL0810250/ KL081001K/ KL105000/ KL080911K)

Please note: all protocols off site are the responsibility of the products supplier

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References

References

 

    1. Feinberg, A.P. and Vogelstein, B. (1984) Anal. Biochem. 137, 266.

 

    1. Sanger, F. et. al. (1977) Proc. Natl. Acad. Sci. USA 74, 5463.

 

    1. Molecular Cloning: A Laboratory Manual (1989), J. Sambrook, E.F. Fritsch and T. Maniatis, editors, Cold Spring Harbor Laboratory Press, 2nd edition, v.2, p. 8.14.

 

  1. Walter, N.G. (1995) J. Mol. Biol. 254, 856.

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Applications & Benefits

Applications

  • Random-primer labeling of DNA.1
  • Second-strand cDNA synthesis.2
  • Strand-displacement amplification.3

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Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200