Cambio - Excellence in Molecular Biology

Enzymes for Molecular Biology

Enzymes for Molecular Biology: Heat labile & Salt Tolerant Enzymes

New range of enzymes for 2019. Our new range of enzymes include novel enzymes specifically designed or engineered to be amazingly tolerant to high salt concentrations, function at very low temperatures and heat inactivated.

Cryophile PCR Decon kit

The Cryophile PCR Decontamination Kit removes contaminating DNA in PCR master mixes, without reduction of PCR sensitivity.

Cambio

Catalogue No.DescriptionPack SizePriceQty
CA-1738-100Cryophile PCR Decon kit100 rxn £80.00 Quantity Add to Order
CA-1738-500Cryophile PCR Decon kit500 rxn £370.00 Quantity Add to Order

Cryophile PCR Decon kit

The Cryophile PCR Decontamination Kit removes contaminating DNA in PCR master mixes, without reduction of PCR sensitivity.

Cambio

The PCR Decontamination Kit removes contaminating DNA in PCR master mixes, without reduction of PCR sensitivity. 

Note: due to the wide variety of master mixes available, we recommend testing your master mixes for compatibility.

•    The PCR Decontamination Kit removes contaminating DNA in PCR master mixes, without reduction of PCR sensitivity.*
•    The double-strand specific property of the dsDNase allows decontamination with primers and probe present.**
•    Efficient for end-point PCRs and probe based qPCR mixes, and for some SYBR based qPCR mixes.
•    Contaminating bacterial DNA is reduced to levels below detection limit.
•    Flat NTCs!
•    The sensitivity of the PCR is not affected.
•    Fast and easy protocol.

      

Kit Contents:


DTT (Inactivation Aid)
Cryophile dsDNase HL65

 

* Reduced PCR sensitivity can be observed in some kits, especially where hot-start is damaged upon inactivation of the dsDNase

*In some cases, primers and probes might form hairpin structures (not necessarily compatible) that might act as a target for the dsDNase. Hence, this must be tested from case to case. If this occurs, primers must be added after the decontamination.

 


If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200