Cambio - Excellence in Molecular Biology

Transfection - EZ Biosystems

Transfection - EZ Biosystems: Primary Cells

Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:?

Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines). Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated. 

For cell lines click HERE

To learn why you should use Avalanche® Cell Type-Specific Transfection reagents, click HERE; To learn how EZ Biosystems developed Avalanche® Cell Type-Specific Transection Reagents, click HERE

Human Prostate Stromal Cell (PrSC) Avalanche™ Transfection Reagent

Transfection of primary cells, such as PrSc, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells. Human Prostate Stromal Cell (PrSC) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on PrSc. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for PrSc. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Prostate Stromal Cell (PrSC) Avalanche™ Transfection Reagent for transfection on PrSc

EZ Biosystems

Catalogue No.DescriptionPack SizePriceQty
EZT-PRSC-1-05Human Prostate Stromal Cell Transfection Reagent0.5ml £364.00 Quantity Add to Order
EZT-PRSC-1-15Human Prostate Stromal Cell Transfection Reagent1.5ml £636.00 Quantity Add to Order

Human Prostate Stromal Cell (PrSC) Avalanche™ Transfection Reagent

Transfection of primary cells, such as PrSc, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells. Human Prostate Stromal Cell (PrSC) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on PrSc. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for PrSc. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Prostate Stromal Cell (PrSC) Avalanche™ Transfection Reagent for transfection on PrSc

EZ Biosystems

Cell Information:

Prostate cells provide a glandular function in the body by generating fluid which serves several functions in reproduction.  

Prostates consist of glandular epithelium embedded in a fibro-muscular stroma. The secretory luminal layer of the epithelium is made up of tall columnar cells that are responsible for the production of PSA, PAP and human kallikrein-2 that are secreted as part of the seminal fluid into the glandular lumina.

Human Prostate Stromal Cells (PrSC) are necessary for the normal growth and survival of epithelial cells in the prostate. A number of findings have suggested interactions between PrSC and the epithelium as well as importance of the extracellular matrix in prostate development and differentiation; alterations of these interactions may lead to prostate cancer progression (Djakiew et al., 1990; Cunha et al., 1996, 2002). Stromal–epithelial interactions appear to be reciprocal, mediated by steroid hormones, soluble and insoluble factors and their receptors, and constituents of the extracellular matrix (Chung, 1993; Rowley, 1999).

PrSC can be used for the following applications:

  • Benign prostatic hyperplasia (BPH)
  • Prostitis
  • Prostate cancer
  • Procreation research
  • Drug discovery
  • Male prostate physiology

The Transfection Reagent:

Transfection of primary cells, such as PrSc, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for cell lines in culture fail to transfect primary cells.

Human Prostate Stromal Cell (PrSC) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on PrSc. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for PrSc. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Prostate Stromal Cell (PrSC) Avalanche™ Transfection Reagent for transfection on PrSc. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

  • Specifically optimized to deliver nucleic acids into PrSc
  • Highest efficiency to ensure experimental success
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases 
  • synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
  • Simplest and straightforward protocols
  • Suitable for Reverse Transfection 
  • Compatible with transfection in any plate formats
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems

 

Data:
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human prostate stromal cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human prostate stromal cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Prostate Stromal Cell Avalanche Transfection Reagent.
 

For Other Cells:
Human Prostate Stromal Cell (PrSC) Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies.

786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200