Cambio - Excellence in Molecular Biology

Transfection - EZ Biosystems

Transfection - EZ Biosystems: Primary Cells

Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:?

Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines). Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated. 

For cell lines click HERE

To learn why you should use Avalanche® Cell Type-Specific Transfection reagents, click HERE; To learn how EZ Biosystems developed Avalanche® Cell Type-Specific Transection Reagents, click HERE

Human Airway Epithelial Cell (HBEpC, HTEpC, NHBE, SAEC) Avalanche™ Transfection Reagent

Transfection of primary cells, such as Human Airway Epithelial Cells, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations. Human Airway Epithelial Cell (HBEpC, HTEpC, NHBE, SAEC) Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting Human Airway Epithelial Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Human Airway Epithelial Cells

EZ Biosystems

Catalogue No.DescriptionPack SizePriceQty
EZT-HBEC-1-05Human Airway Epithelial Cell Transfection Reagent0.5ml £364.00 Quantity Add to Order
EZT-HBEC-1-15Human Airway Epithelial Cell Transfection Reagent1.5ml £636.00 Quantity Add to Order

Human Airway Epithelial Cell (HBEpC, HTEpC, NHBE, SAEC) Avalanche™ Transfection Reagent

Transfection of primary cells, such as Human Airway Epithelial Cells, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations. Human Airway Epithelial Cell (HBEpC, HTEpC, NHBE, SAEC) Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting Human Airway Epithelial Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Human Airway Epithelial Cells

EZ Biosystems

ell Information:

Airway epithelial cells are targets in asthma, viral infection and fibrotic lung disease. Airway epithelial cells include human Bronchial, Tracheal, and Small Airway Epithelial Cells. These cells have been used to study the regulation of intracellular pH, the stimulant effect of IL-1b on airway epithelial cell growth, and the effect of human rhinovirus infection in terms of upregulation of ICAM-1 expression on epithelial cells.

Applications:

  • Asthma
  • COPD
  • Airborne viruses
  • Allergies
  • Inhalation of nanoparticles
  • Cancer
  • Cystic Fibrosis
  • DoD studies

The Transfection Reagent:

Transfection of primary cells, such as Human Airway Epithelial Cells, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.

Human Airway Epithelial Cell (HBEpC, HTEpC, NHBE, SAEC) Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting Human Airway Epithelial Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for Human Airway Epithelial Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent? series?

Features:

  • Specifically optimized to deliver nucleic acids into Human Airway Epithelial Cells
  • Highest efficiency to ensure experimental success
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
  • Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
  • Simplest and straightforward protocols
  • Compatible with serum
  • Suitable for Reverse Transfection 
  • Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems
Data:
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human bronchial epithelial cells (HBEpC) after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human bronchial epithelial cells (HBEpC). This test result was also confirmed by transfecting other airway epithelial Cell mentioned above. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Airway Epithelial Cell Avalanche Transfection Reagent.
 

For Other Cells:
Human Airway Epithelial Cell (HBEpC, HTEpC, NHBE, SAEC) Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies.

Human Renal Epithelial cell (HRE)
Human Prostate Epithelial Cell (HPrEpC)
Human Bladder Epithelial Cell (HBlEpC)
Human Placental Epithelial Cell (HPlEpC)
Human Mammary Epithelial Cell (HMEpC, HMEC)
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200