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Enzymes for Molecular Biology

Enzymes for Molecular Biology: Enzymes for Molecular Biology

RNase I, E. coli

RNase I E. coli degrades single-stranded RNA to nucleoside 3'-monophosphates via 2', 3' cyclic monophosphate intermediates by cleaving every phosphodiester bond

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
N6901KRNase I10 U/µl 1,000U £86.00 Quantity Add to Order

RNase I, E. coli

RNase I E. coli degrades single-stranded RNA to nucleoside 3'-monophosphates via 2', 3' cyclic monophosphate intermediates by cleaving every phosphodiester bond

BioSearch Tech (Lucigen/Epicentre)

RNase I degrades single-stranded RNA to nucleoside 3'-monophosphates via 2', 3' cyclic monophosphate intermediates by cleaving every phosphodiester bond. RNase I completely degrades RNA, unlike RNase A that cleaves only after cytosine and uridine. In addition, the enzyme is completely inactivated by heating at 70°C for 20 minutes, eliminating the requirement to remove the enzyme prior to many subsequent procedures. 

RNase I e coli
Figure 1. Removal of RNA from plasmid DNA preparations. Plasmid DNA was prepared from 1.5 ml of an overnight culture, suspended in 50 µl TE buffer (10 mM Tris-HCl (pH 8), 1 mM EDTA), and treated with RNase A, RNase I, or no enzyme. Five µl of each reaction were resolved by agarose gel electrophoresis and visualized by staining with ethidium bromide. Lane 1, no RNase; Lane 2, 1.5 U of RNase I; Lane 3, 20 µg/ml RNase A; MW, 1-kb ladder. Arrow denotes small undigested RNA.

Unit Definition

One unit degrades 100ng of E. coli ribosomal RNA per second into acid-soluble nucleotides at 37°C in 10mM Tris-HCl, pH 7.5, 100mM NaCl, and 1mM EDTA. 

Dilution and Storage Buffers

50mM Tris-HCl, pH 7.5, 100mM NaCl, 0.1mM EDTA in 50% glycerol. 

Quality Control

RNase I is free of detectable DNA exo- and endonuclease activities.

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RNase I, E. coli

RNase I E. coli degrades single-stranded RNA to nucleoside 3'-monophosphates via 2', 3' cyclic monophosphate intermediates by cleaving every phosphodiester bond

BioSearch Tech (Lucigen/Epicentre)

Protocols for: RNase I, E. coli 

RNase I Protocol

(catalogue number N6901K / N6905k) 

Please note: all protocols off site are the responsibility of the products supplier

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RNase I, E. coli

RNase I E. coli degrades single-stranded RNA to nucleoside 3'-monophosphates via 2', 3' cyclic monophosphate intermediates by cleaving every phosphodiester bond

BioSearch Tech (Lucigen/Epicentre)

References

 

  1. Shen, V. and Schlessinger, D. (1982) The Enzymes XV (Part B), 501.
  2. delCardayré, S.B. and Raines, R.T. (1995) Anal. Biochem. 225, 176.
  3. Johnson, M.G. (1996) EPICENTRE Forum 3 (4), 7.

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RNase I, E. coli

RNase I E. coli degrades single-stranded RNA to nucleoside 3'-monophosphates via 2', 3' cyclic monophosphate intermediates by cleaving every phosphodiester bond

BioSearch Tech (Lucigen/Epicentre)

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RNase I, E. coli

RNase I E. coli degrades single-stranded RNA to nucleoside 3'-monophosphates via 2', 3' cyclic monophosphate intermediates by cleaving every phosphodiester bond

BioSearch Tech (Lucigen/Epicentre)

Applications

  • Removal of RNA from DNA preparations.1
  • RNase protection assays to detect single-basepair mismatches in RNA:RNA and RNA:DNA hybrids.1

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