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Enzymes for Molecular Biology

Enzymes for Molecular Biology: Enzymes for Molecular Biology

Baseline-ZERO™ DNase

Baseline-ZERO&trade digests double- and single-stranded DNA to mononucleotides more effectively than the commonly used bovine pancreatic DNase I

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
DB0715KBaseline-ZERO™ DNase5,000MBU £204.00 Quantity Add to Order

Baseline-ZERO™ DNase

Baseline-ZERO&trade digests double- and single-stranded DNA to mononucleotides more effectively than the commonly used bovine pancreatic DNase I

BioSearch Tech (Lucigen/Epicentre)

Effectively digest large or small dsDNA and ssDNA into mononucleotides

  • Digest unwanted dsDNA and ssDNA molecules including small ssDNA such as random hexamers
  • Use in sensitive applications requiring reverse transcription where any contaminating DNA is unwanted

 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Baseline-ZERO™ DNase

Baseline-ZERO&trade digests double- and single-stranded DNA to mononucleotides more effectively than the commonly used bovine pancreatic DNase I

BioSearch Tech (Lucigen/Epicentre)

Protocols for: Baseline-ZERO™ DNase

Due to the constant updating of the protocols by the manufacturer we have provided a direct link to Lucigens’s product page, where the latest protocol is available.

Please note this will open a new page or window on your computer.

 

 Baseline-ZERO™ DNase Protocol

(catalogue number DB0711K, DB0715K)

Please note: all protocols off site are the responsibility of the products supplier

 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Baseline-ZERO™ DNase

Baseline-ZERO&trade digests double- and single-stranded DNA to mononucleotides more effectively than the commonly used bovine pancreatic DNase I

BioSearch Tech (Lucigen/Epicentre)

References

  1.  Kienzle, N. et al., (1996) BioTechniques 20, 612.
  2. Clauson, C. L., et al. (2010) Abasic sites and strand breaks in DNA cause transcriptional mutagenesis in Escherichia coli, PNAS 107 , 3657-3662.
  3. Davie, J. J. & Campagnari, A. A. (2009) Comparative Proteomic Analysis of the Haemophilus ducreyi Porin-Deficient Mutant 35000HP::P2AB, J. Bacteriol. 191 , 2144-2152.
  4. Rungrassamee, W., et al. (2009) The PqrR Transcriptional Repressor of Pseudomonas aeruginosa Transduces Redox Signals via an Iron-Containing Prosthetic Group, J. Bacteriol. 191 , 6709-6721.
  5. Sakayori, T., et al. (2009) A Synechocystis Homolog of SipA Protein, Ssl3451, Enhances the Activity of the Histidine Kinase Hik33, Plant Cell Physiol. 50 , 1439-1448.
  6. Schroeckh, V., et al. (2009) Intimate bacterial-fungal interaction triggers biosynthesis of archetypal polyketides in Aspergillus nidulans, PNAS 106 , 14558-14563.
  7. Victoria, J. G., et al. (2009) Metagenomic Analyses of Viruses in Stool Samples from Children with Acute Flaccid Paralysis, J. Virol. 83 , 4642-4651.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Baseline-ZERO™ DNase

Baseline-ZERO&trade digests double- and single-stranded DNA to mononucleotides more effectively than the commonly used bovine pancreatic DNase I

BioSearch Tech (Lucigen/Epicentre)

Applications

  • Removal of genomic DNA from small-sample total RNA preparations for expression analysis (Fig. 1)
  • Removal of small DNA oligonucleotides (e.g., random primers)

Applications

  • Removal of genomic DNA from small-sample total RNA preparations for expression analysis (Fig. 1)
  • Removal of small DNA oligonucleotides (e.g., random primers)

Baseline-ZERO™ DNase* digests dsDNA and ssDNA into mononucleotides more effectively than the commonly used bovine pancreatic DNase I. Even the small DNA oligonucleotides that remain after treatment with bovine pancreatic DNase I are undetectable by gel electrophoresis following treatment with Baseline-ZERO DNase (Fig. 2). Removal of DNA from RNA preparations is particularly beneficial when RNA in a sample is amplified using a method that involves reverse transcription using random primers, since any contaminating DNA would also be a template for random-primed cDNA synthesis.

Figure 1. Real-time PCR of HeLa RNA preparations treated with various DNases. The lower the CT value (intersection of curves with the red line), the greater the amount of residual DNA not digested by the indicated DNase. Thus, Baseline-ZERO™ DNase removed all detectable DNA from the RNA sample. The TaqMan® probe assay amplified a 268-bp fragment of β-actin. Samples were run in duplicate.

 

Unit Definition: One Molecular Biology Unit (MBU) of Baseline-ZERO™ DNase produces an increase in the A260 of a solution of dsDNA, of 0.001 per minute at 25°C. Functionally, 1 MBU completely digests 1 µg of linear pUC19 DNA to mononucleotides in 10 minutes at 37°C.

Storage Buffer: Baseline-ZERO DNase is supplied in a 50% glycerol solution containing 50 mM Tris-HCl (pH 7.5), 10 mM CaCl2, 10 mM MgCl2 and 0.1% Triton® X-100.

10X Baseline-ZERO™ DNase Reaction Buffer: 100 mM Tris HCl (pH 7.5), 25 mM MgCl2 and 5 mM CaCl2.

10X Baseline-ZERO™ DNase Stop Solution: 30 mM EDTA.

Quality Control: Baseline-ZERO DNase is assayed for its ability to completely digest linear dsDNA to mononucleotides under standard assay conditions. Baseline-ZERO DNase is free of detectable RNase activities as assayed by PAGE analysis of 1 µg of a synthetic RNA transcript following an overnight incubation with sufficient DNase I to completely digest 1,000 µg of DNA.

References

Kienzle, N. et al. (1996) BioTechniques 20, 612

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200