The CopyControl Autoinduction Solutions are designed to provide a simpler way to induce CopyControl BAC and Fosmid vectors to high copy number. The autoinduction solution, which is added prior to culture inoculation, is designed to cause induction to occur prior to DNA purification without the need for sub-culturing. The simple, hands-off protocol is particularly useful in high throughput purifications where sub-culturing is tedious and inefficient.
In addition to the simpler induction protocol, CopyControl Autoinduction Solutions also contain ingredients which enhance cell growth, leading to higher culture ODs, and subsequently higher DNA yields than are typically seen using the standard CopyControl Induction Solution.
The two Autoinduction Solutions are cloning-system-specific and not interchangeable, so the proper Autoinduction Solution must be used with each cloning system, as indicated, for optimum results
*Covered by issued and/or pending patents
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Figure 1. Uninduced (single-copy)(-) and autoinduced( ) CopyControl™ BACs purified using the BACMAX96™ DNA purification system. When used in conjunction with the BACMAX96 system, autoinduced CopyControl vectors typically yield over 3 µg of DNA using 1.2 ml of starting culture. |
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