ExgeneTM Blood SV kit provides a simple and rapid method for the isolation of total DNA from fresh or frozen whole blood, buffy coat, serum, plasma, virus and cultured cells. Purification procedure is so simple and
optimized to simultaneous processing of multiple samples. ExgeneTM Blood SV yields pure DNA ready for direct PCR in just 20 minutes (mini) and 1 hour (Midi / MAXI). There is no need phenol extraction or alcohol precipitation.
Features and Benefits
¦ Spin or vacuum column format
¦ Accurate and consistent DNA extraction from whole blood, buffy coat, serum, plasma,cultured cells
¦ Instant use : No need of additional materials
¦ Fast, safe and simple procedure completed in 20 minutes (mini), 1 hour (Midi, MAXI)
¦ High purity : 1.8 ~ 2.0
¦ No use of organic solvents
¦ Ready for use in PCR, southern blotting and other enzymatic reactions
Protocol for Exgene Blood SV mini
Protocol for Exgene Blood SV Midi/Maxi
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| Features |
Blood SV mini |
Blood SV Midi |
Blood SV MAXI |
|---|
| Format |
Column type G (mini) with collection tube | Column type G (Midi) with collection tube | Column type G (MAXI) with collection tube |
| Maximum sample size | ~200 μl | 1~2 ml | 3~10 ml
|
| Preparation time | 20~30 minutes | 40~55 minutes | 40~55 minutes |
| Typical yield | 4~20 μg | 20~80 μg | 80~400 μg |
| Elution volume | 30~400 μl | 200~600 μl | 0.4~2 ml |
Technical Data
Genomic DNA Extraction from Various Samples
Compatibility Test with Restriction Enzymes
PCR Amplification Test
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200