Applications

Extraction & Purification - DNA: Plant

Specialised kits for isolating DNA from plant materials such as plant leaf, seeds, roots and stem.

QuickExtract™ Plant DNA Extraction Solution

The QuickExtract™ Plant DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from most plant leaf samples.

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.	Description	Pack Size	Price	Qty	Change to: €
QEP70750	QuickExtract™ Plant DNA Extraction System	500 Reactions	£362.00	Quantity	Add to Order
QEP70750-PQ1497-A	10X QuickExtract™ Plant DNA Extraction Solution	5ml	£382.00	Quantity	Add to Order
QEP70750-PQ1497-B	10X QuickExtract™ Plant DNA Extraction Solution	50ml	POA	Quantity	Add to Order

Description

The QuickExtract™ Plant DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from most plant leaf samples using a simple, one-tube protocol that takes only 8 minutes (Fig. 1). Most leafy plants are suitable for DNA extraction using the QuickExtract Plant Solution, including Arabidopsis, barley, maize, emmer, pepper, rice, spelt, spinach, soybeans, and wheat (e.g., Fig. 2).

The QuickExtract Plant method allows for the inexpensive processing of one to hundreds of samples simultaneously, without grinding the sample, centrifugation, spin columns, or any toxic organic solvent. The procedure is fully compatible with robotic automation, provides a PCR-ready sample, and is reproducible (Fig. 3). Simply add the QuickExtract Plant solution to the sample and perform two sequential heating steps. A small aliquot of the sample is then used as a template for PCR or qPCR.

Figure 1. Overview of the QuickExtract™ Plant DNA extraction procedure.

Figure 2. PCR products using QuickExtract™ Plant DNA Extraction Solution with different varieties of plant leaves. Forty cycles of RAPD were performed with DNA extracted from plant leaves using the QuickExtract Plant solution and the FailSafe™ PCR System. Lane M, 100-bp ladder; lane 1, pepper; lane 2, soybean; lane 3, spelt.

Figure 3. Reproducibility of PCR results with QuickExtract™ Plant DNA Extraction Solution from Arabidopsis thaliana leaves. Six individual punches of four Arabidopsis leaves were treated with QuickExtract Plant solution as described in the product information sheet. One microliter of the solution was used in a 25-µl PCR using the FailSafe™ PCR System and primers specifi c for the single-copy HSC70 chromosomal gene. Aliquots were analyzed on a 2% agarose gel and the DNA was visualized by staining with SYBR® Gold. Lanes 1-6, PCR products of extracted DNA samples; lane 7, no-leaf negative control; lane M, 100-bp DNA ladder. The expected amplicon is approximately 520 bp