Cambio - Excellence in Molecular Biology

Cell Culture Contamination Control

Cell Culture Contamination Control: Detection

Mycoplasma Detection

As an efficient contamination control measure we recommend frequent routine testing of cell culture materials with Venor®Gem Mycoplasma Detection Kits. These PCR- and qPCR-based mycoplasma assays allow fast (within a few hours), simple, effective and sensitive detection of mycoplasma-contaminated cell cultures in any standard molecular biology laboratory.

Regular mycoplasma testing and control procedures can significantly help in avoiding the costly consequences of contamination ensuring safe biopharmaceuticals and reliable scientific results.  Many scientific journals currently do not accept submissions of cell-based studies without evidence that mycoplasma testing has been performed.

To meet different customer needs Minerva Biolabs has developed a range of Venor®GeM mycoplasma detection assays using conventional PCR or qPCR methods which enable rapid and reliable identification of mycoplasma in cell cultures, culture supernatants and tissue culture media.

Conventional PCR

  • Venor®GeM Classic allows fast, reliable and time-saving routine monitoring of mycoplasma contamination in cell culture supernatants, media and biopharmaceuticals in research and industry by conventional PCR.

  • Venor®GeM OneStep is a kit for direct detection. It requires minimal pipetting, the kit includes all reagents required for PCR in a ready-to-use lyophilized reaction mix. Only samples or controls need to be added prior to PCR.

  • Venor®GeM Advance is designed for direct detection. Designed to reduce the total assay time and the pipetting steps, the kit contains PCR strips of tubes, each pre-dispensed with all reagents necessary for each PCR reaction, including the polymerase. For additional convenience, the gel loading buffer and dye are already included in the reaction buffer. After PCR, the products can be loaded directly on an agarose gel.

Real-time PCR (qPCR)

Our qPCR kits are TaqMan®-based qPCR Assays with labelled probes:  FAM® channel (mycoplasma) and HEX® channel (internal control to check for PCR inhibition from contaminants in sample).  So not only does the qPCR approach remove a lot of hassle, but the internal controls also give you reassurance that your negatives are not false.

  • Venor®GeM qEP utilises quantitative, real-time PCR (qPCR) for fast (approx. 3 h) and reliable screening of cell culture supernatants for mycoplasma contamination. The kit can be used in combination with any type of real-time PCR cycler able to detect the fluorescence dyes FAM® and HEX®. For clinical diagnostics in which human samples are tested we recommend using this kit as it conforms to EP 2.6.7, USP <71> and JP G3. It is suitable for both research and industry where certified compliance is needed or desirable. 

  • Venor®GeM qOneStep is a kit based on a real-time PCR (qPCR) assay for rapid, robust and sensitive detection of mycoplasma contamination. The detection procedure can be performed within 3 hours. Primers, nucleotides, probes, polymerase and an internal amplification control are provided as a ready-to-use, lyophilized reaction mix. This kit is a very straightforward and is highly suitable for academic labs and industrial applications where certified compliance of kits is not required.


MycoSPY™

MycoSPY™ PCR kit for Rapid Mycoplasma Detection from Biontex

Biontex

Catalogue No.DescriptionPack SizePriceQty
M030-010MycoSPY™10 Assays £203.00 Quantity Add to Order
M030-025MycoSPY™25 Assays £280.00 Quantity Add to Order
M030-050MycoSPY™50 Assays £246.00 Quantity Add to Order

Description

A PCR-Based Mycoplasma Detection Kit 

Several methods are available for the detection of mycoplasmas. However, the best results in routine detection of mycoplasmas can only be achieved by Polymerase Chain Reaction (PCR)-based methods, which give reliable, fast and sensitive results. 

Biontex has now developed MycoSPY™, a new PCR-based detection kit for application in your routine cell culture work.

In contrast to other commercially available kits based on enzymatic tests, MycoSPY™ can shorten the duration of tests from 4 to 5 days down to 3 hours!  

MycoSPY™ PCR primers are derived from consensus sequences by multiple alignments of 16S rRNA genes of mycoplasma strains. Only a mixture of primers guarantees detection of almost every known mycoplasma species. 

List of Mollicute strains detected with PCR primer from MycoSpy™ Kit
List of Mollicute strains detected with PCR primer from MycoSpy™ Kit
Mycoplasma
M. alligatoris M. cricetuli M. hypopneumoniae M. phoacae
M. alvi M. crocodyli M. imitans M. phocicerebrale
M. amphiforme M. cynis M. iowae M. pirum
M. arginii M. dispar M. lacerti M. pneumoniae
M. bovigenitalum M. edwardii M. lagogenitalum M. sualvi
M. buccale M. elephantis M. microti M. synoviae
M. canadense M. equigenitalum M. moatsii M. testudineum
M. canimucosale M. felis M. molare M. testudinis
M. canis M. gallisepticum M. muris M. verecundum
M. capricolum M. genitalium M. mustelae M. volis
M. cavipharyngis M. haemaofelis M. mycoides M. yeatsii
M. citelli M. hominis M. orale M. zalophi
M. columborale M. hyorhinis M. oxoniensis
M. conjunctivae M. hyosynoviae M. penetrans
Ureaplasma
U. cangenitalium U. diversum U. gallorale U. parvurm
U. cati U. felinum U. loridis U. urealyticum
Mesoplasma
M. chaulicola M. florum M. photuris M. tabanidae
M. entomobilium M. grammopterae M. syrphidae
Spiroplasma
S. cantharicola S. lineolae S. taiwanense
S. citri S. platyhelix
Acholeplasma
A. laidlawii
Phytoplasma
P. spp

 

Product Specifications 
Application PCR kit for detection of mycoplasmas in cell culture
Content Taq polymerase, MycoSPY™ Primer Mix, MycoSPY™ internal control and taq polymerase buffer with nucleotide triphosphates (dNTPs)
Assays 10, 25, or 50 applications per kit
Sensitivity < 50 cfu/ml mycoplasmas
Shipping In dry ice
Storage ≤ –15°C
Shelf Life 1 year (after date of delivery)

The MycoSpy™ kit contains a complete range of reagents for reliable and sensitive PCR. It is available in three sizes sufficient for 10, 25 or 50 assays. Each kit contains Taq Polymerase, MycoSpy™ Primer Mix, MycoSpy™ Internal Control and Taq Polymerase Buffer with nucleotides.

 


If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Applications & Benefits

Application

  • PCR kit for rapid, highly sensitive detection of mycoplasmas

Benefits

    • Easy Protocol
    • Highest safety
    • Fast results in less than 3 hours
    • Broad efficiency range
    • Sensitive (Detects <50cfu/ml mycoplasma)
    • Cost-effective
    • Complete kit system

 

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200