The TATAA Interplate Calibrator (IPC) makes it possible for you
to compensate for variations between qPCR runs. For practical reasons
many qPCR studies involve the use of samples that are processed in more
than a single batch or in which the sample set is extended over time.
Even over a short time period, variation between qPCR processing runs is
observed due to different baseline subtractions and threshold settings.
When to use TATAA Interplate Calibrator
The bias is NOT introduced when using the “all samples” or “all
assays” plate layout or when performing ΔΔCq based analysis, but it is
the “mixed” layout for which interplate calibration is needed (Figure
1). The TATAA IPC sample material is provided in ready-to-use aliquots
and is a very stable template that is amplified with a highly robust
assay. The TATAA IPC should be included in all qPCR runs. Any
differences in the measured IPC Cq-values among the runs reflect the
bias introduced by the instrument and can then compensated for.
TATAA Interplate Calibrator is:
- provided in aliquots (stored at -20°C) for easy and flexible use and long term stability
- a robust assay that performs excellent in most mastermixes and over a wide range of annealing temperatures
- a stable template at optimum concentration that produces Cq ≈ 15 -20 under most conditions